Azov N A, Maianskiĭ A N
Zh Mikrobiol Epidemiol Immunobiol. 1985 Oct(10):66-9.
The opsonic properties of normal serum with respect to E. coli peptidoglycan was studied under the actual conditions of the oxygen-dependent metabolism of neutrophils. In the course of the differentiated study of the influence of antibodies, the classical and the alternative cascades of complement the serum was heated, treated with ethylenediaminetetraacetate and ethylene glycol tetraacetate, exhausted in the cold. In serial experiments the opsonic activity of purified fibronectin was studied. The indirect reactions were shown to be the leading mechanisms of the neutrophil-stimulated activity of E. coli peptidoglycan. IgG was found to be in the center of the opsonic cooperation and thus to determine the quantitative manifestation of the total phenomenon. Complement proved to be of lesser importance; depending on the conditions of the experiment, the activation of complement occurred by the alternative way (after the removal of antibodies) or the classical way (whole serum). The actual contribution of IgG-independent and complement-independent opsonins was insignificant. Fibronectin in physiological concentrations showed no opsonic activity.
在中性粒细胞氧依赖代谢的实际条件下，研究了正常血清对大肠杆菌肽聚糖的调理特性。在对抗体、经典补体途径和替代补体途径影响的差异化研究过程中，血清经过加热、用乙二胺四乙酸和乙二醇四乙酸处理，并在低温下耗尽。在系列实验中，研究了纯化纤连蛋白的调理活性。结果表明，间接反应是大肠杆菌肽聚糖刺激中性粒细胞活性的主要机制。发现IgG处于调理协同作用的中心，从而决定了整个现象的定量表现。补体的重要性较低；根据实验条件，补体的激活通过替代途径（去除抗体后）或经典途径（全血清）发生。不依赖IgG和不依赖补体的调理素的实际贡献微不足道。生理浓度的纤连蛋白未表现出调理活性。
