Ma Jingze, Fan Junjun, Zhang Wangxiang, Zhou Ruomiao, Shen Yiting, Peng Qin, Li Huimin, Lei Cong
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China.
Plants (Basel). 2024 Jul 26;13(15):2080. doi: 10.3390/plants13152080.
'Baiyun' (registration no. 20210210), a new crabapple cultivar, was registered in 2021 by the Nanjing Forestry Unversity. However, the difficult rooting has greatly limited the production of high-quality . 'Baiyun' in industrialization development. There is thus a pressing need to develop an organogenesis protocol for the in vitro propagation of 'Baiyun' to alleviate a shortage of high-quality 'Baiyun' seedlings. The results showed that choosing the apical bud in mid-March was an excellent explant material. To promote proliferation, the highest proliferation (6.27) of apical shoots was cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg·L 6-benzylaminopurine(6-BA) + 0.05 mg·L indole-3-butyric acid (IBA). Subsequently, a 100% rooting rate, average number of roots per shoot of 6.2 and maximum length of roots of 4.96 cm were obtained on half-strength Murashige and Skoog (1/2 MS) medium with the application of 0.5 mg·L naphthaleneacetic acid (NAA) or 0.6 mg·L NAA + 0.7 mg·L IBA. Additionally, thick and lateral roots were obtained with 0.6 mg·L NAA + 0.7 mg·L IBA. Our study is the first to establish an effective organogenesis protocol for new crabapple cultivars using stem segments.
‘白云’（登记号：20210210）是一种新的海棠品种，于2021年由南京林业大学登记注册。然而，生根困难极大地限制了优质‘白云’在产业化发展中的生产。因此，迫切需要开发一种用于‘白云’离体繁殖的器官发生方案，以缓解优质‘白云’幼苗的短缺。结果表明，选择3月中旬的顶芽是优良的外植体材料。为促进增殖，将顶芽培养在添加了0.5 mg·L 6-苄基腺嘌呤（6-BA）+ 0.05 mg·L吲哚-3-丁酸（IBA）的Murashige和Skoog（MS）培养基上时，顶芽的增殖率最高（6.27）。随后，在添加了0.5 mg·L萘乙酸（NAA）或0.6 mg·L NAA + 0.7 mg·L IBA的1/2强度Murashige和Skoog（1/2 MS）培养基上，生根率达到100%，每株苗的平均生根数为6.2，根的最大长度为4.96 cm。此外，使用0.6 mg·L NAA + 0.7 mg·L IBA可获得粗壮的侧根。我们的研究首次利用茎段为新的海棠品种建立了有效的器官发生方案。
