Milenković Strahinja, Virijević Katarina, Živić Fatima, Radojević Ivana, Grujović Nenad
Institute for Information Technologies, University of Kragujevac, 34000 Kragujevac, Serbia.
Faculty of Engineering, University of Kragujevac, 34000 Kragujevac, Serbia.
Materials (Basel). 2024 Aug 5;17(15):3872. doi: 10.3390/ma17153872.
This study focused on preparing composite nanomats by incorporating silver nanoparticles (AgNPs) in polyvinylidene fluoride (PVDF) nanofibers through the electrospinning process. A short review of piezoelectric PVDF-related research is presented. PVDF is known for its biocompatibility and piezoelectric properties. Since electrical signals in biological tissues have been shown to be relevant for therapeutic applications, the influence of the addition of AgNPs to PVDF on its piezoelectricity is studied, due to the ability of AgNPs to increase the piezoelectric signal, along with providing antibacterial properties. The prepared samples were characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. In addition, the biological activity of composites was examined using a cytotoxicity assay and an assessment of the antibacterial activity. The obtained results show that the incorporation of AgNPs into PVDF nanofibers further enhances the piezoelectricity (crystalline β-phase fraction), already improved by the electrospinning process, compared to solution-casted samples, but only with a AgNPs/PVDF concentration of up to 0.3%; a further increase in the nanoparticles led to a β-phase reduction. The cytotoxicity assay showed a promising effect of PVDF/AgNPs nanofibers on the MDA-MB-231 breast cancer cell line, following the non-toxicity displayed in regard to the healthy MRC-5 cell line. The antibacterial effect of PVDF/AgNPs nanofibers showed promising antibacterial activity against and , as a result of the Ag content. The anticancer activity, combined with the electrical properties of nanofibers, presents new possibilities for smart, multifunctional materials for cancer treatment development.
本研究聚焦于通过静电纺丝工艺将银纳米颗粒（AgNPs）掺入聚偏氟乙烯（PVDF）纳米纤维中来制备复合纳米垫。本文对与压电PVDF相关的研究进行了简要综述。PVDF以其生物相容性和压电性能而闻名。由于生物组织中的电信号已被证明与治疗应用相关，鉴于AgNPs具有增强压电信号以及提供抗菌性能的能力，因此研究了向PVDF中添加AgNPs对其压电性的影响。通过扫描电子显微镜、能量色散X射线光谱和傅里叶变换红外光谱对制备的样品进行了表征。此外，使用细胞毒性试验和抗菌活性评估来检测复合材料的生物活性。所得结果表明，与溶液浇铸样品相比，将AgNPs掺入PVDF纳米纤维中进一步增强了已经通过静电纺丝工艺提高的压电性（结晶β相分数），但仅在AgNPs/PVDF浓度高达0.3%时如此；纳米颗粒的进一步增加导致β相减少。细胞毒性试验表明，PVDF/AgNPs纳米纤维对MDA - MB - 231乳腺癌细胞系具有良好的效果，同时对健康的MRC - 5细胞系显示出无毒性。由于Ag的含量，PVDF/AgNPs纳米纤维的抗菌作用对[具体细菌1]和[具体细菌２]显示出良好的抗菌活性。抗癌活性与纳米纤维的电学性质相结合，为癌症治疗开发的智能、多功能材料提供了新的可能性。
