Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Int J Mol Sci. 2024 Jul 28;25(15):8231. doi: 10.3390/ijms25158231.
The hair follicle is the basis of hair regeneration, and the dermal papilla is one of the most important structures in hair regeneration. New intervention and reversal strategies for hair loss may arise due to the prevention of oxidative stress. GC/MS analysis was used to determine the compounds contained in NSO. Then, NSO was applied to DPC for cell proliferation and oxidative stress experiments. RNA-seq was performed in cells treated with NSO and minoxidil. The quantitative real-time polymerase chain reaction (qRT-PCR) was applied to verify the gene expression. The effects of NSO on hair length, weight, the number and depth of hair follicles, and the dermal thickness were also studied. GC/MS analysis showed that the main components of NSO were eicosapentaenoic acid, palmitic acid, and linoleic acid. NSO promotes DPC proliferation and reduces HO-mediated oxidative damage. NSO can also activate hair growth-related pathways and upregulate antioxidant-related genes analyzed by gene profiling. The topical application of NSO significantly promotes hair growth and increases hair length and weight in mice. NSO extract promotes hair growth and effectively inhibits oxidative stress, which is beneficial for the prevention and treatment of hair loss.
毛囊是头发再生的基础，而真皮乳头是头发再生最重要的结构之一。由于氧化应激的预防，可能会出现新的脱发干预和逆转策略。GC/MS 分析用于确定 NSO 中含有的化合物。然后，将 NSO 应用于 DPC 进行细胞增殖和氧化应激实验。对用 NSO 和米诺地尔处理的细胞进行 RNA-seq 分析。应用定量实时聚合酶链反应 (qRT-PCR) 验证基因表达。还研究了 NSO 对毛发生长、重量、毛囊数量和深度以及真皮厚度的影响。GC/MS 分析表明，NSO 的主要成分是二十碳五烯酸、棕榈酸和亚油酸。NSO 促进 DPC 增殖并减少 HO 介导的氧化损伤。NSO 还可以通过基因谱分析激活与头发生长相关的途径并上调抗氧化相关基因。NSO 的局部应用可显著促进毛发生长，增加小鼠的毛发生长长度和重量。NSO 提取物促进头发生长并有效抑制氧化应激，有利于脱发的预防和治疗。
