Thangaraj Priyanka, Sakthignanavel Arulmozhi, Kaliyamoorthy Siva, Thingujam Sonee, Radhakrishnan Vaishnav
Department of Otorhinolaryngology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission Research Foundation, Deemed to be University, Puducherry, India.
Department of Pathology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission Research Foundation, Deemed to be University, Puducherry, India.
Indian J Otolaryngol Head Neck Surg. 2024 Aug;76(4):3001-3006. doi: 10.1007/s12070-024-04569-z. Epub 2024 Mar 15.
The objective of the study was to detect the presence of Helicobacter pylori in adenotonsillar tissue and to assess the association between the presence of H pylori with Chronic Tonsillitis or Adenotonsillitis. This was a cross sectional study conducted among 60 patients diagnosed with chronic tonsillitis and adeno tonsillitis undergoing tonsillectomy or adeno tonsillectomy meeting the paradise criteria in a tertiary care hospital, Pondicherry. Rapid urease test was done in the intraoperative period immediately after the specimens were taken.The rapid urease test kit was observed for color change from yellow to pink within 4 h. The tissue was sent for histopathological examination for staining with H&E and Giemsa stain to detect the presence of helicobacter pylori. The mean age of the study participants was 15.75 ± 8.46 and majority of the study participants were females. (61.7%). 66.7% and 33.3% of the study participants had chronic tonsillitis and adeno tonsillitis respectively. Oral swab showed normal flora, yeast and methicillin resistant Staphylococcus aureus in 96.6%, 1.7% and 1.7% respectively. 1.7% (1),13.3% (8) and 20% (12) of the study participants showed positive in card test immediate, at 15 min and 1 h respectively. Giemsa stain showed that 11.7% was positive for H.pylori. Out of the 7 patients with positive Giemsa stain, 4 had chronic tonsillitis and 3 had adenotonsillitis. Colonisation of adenoids and tonsils by H. pylori is a novel forefront with contradictory results dependent on the precision of the detective techniques used and population studied. Further research may be warranted to establish the varied colonisation depending on the geographical locations.
本研究的目的是检测腺样体扁桃体组织中幽门螺杆菌的存在情况，并评估幽门螺杆菌的存在与慢性扁桃体炎或腺样体扁桃体炎之间的关联。这是一项横断面研究，在本地治里一家三级护理医院中，对60例被诊断为慢性扁桃体炎和腺样体扁桃体炎且符合天堂标准并接受扁桃体切除术或腺样体扁桃体切除术的患者进行。在术中取出标本后立即进行快速尿素酶试验。观察快速尿素酶试验试剂盒在4小时内颜色从黄色变为粉红色的情况。将组织送去进行组织病理学检查，用苏木精-伊红（H&E）和吉姆萨染色以检测幽门螺杆菌的存在。研究参与者的平均年龄为15.75±8.46岁，大多数研究参与者为女性（61.7%）。分别有66.7%和33.3%的研究参与者患有慢性扁桃体炎和腺样体扁桃体炎。口腔拭子显示，正常菌群、酵母菌和耐甲氧西林金黄色葡萄球菌的检出率分别为96.6%、1.7%和1.7%。分别有1.7%（1例）、13.3%（8例）和20%（12例）的研究参与者在即刻、15分钟和1小时的卡式试验中呈阳性。吉姆萨染色显示11.7%的幽门螺杆菌呈阳性。在吉姆萨染色呈阳性的7例患者中，4例患有慢性扁桃体炎，3例患有腺样体扁桃体炎。幽门螺杆菌在腺样体和扁桃体中的定植是一个新的前沿领域，其结果相互矛盾，这取决于所使用的检测技术的准确性和所研究的人群。可能需要进一步研究以确定根据地理位置的不同定植情况。
