Dutta Utpal, Goswami Manab Jyoti, Seema Tage, Payum Temin, Ullah Tanzim Nishad, Hui Pallabi Kalita, Kakati Dwipen
Department of Chemistry, Rajiv Gandhi University, Rono Hills, Doimukh, Arunachal Pradesh 791112, India.
Department of Botany, Jawaharlal Nehru College, Hill Top, Pasighat, Arunachal Pradesh 791103, India.
Heliyon. 2024 Jul 14;10(14):e34638. doi: 10.1016/j.heliyon.2024.e34638. eCollection 2024 Jul 30.
The plant based natural products have always been a rich source of bioactive molecules for drug discovery. The tender shoots of Griff., an edible medicinal plant was extracted using methanol, water and ethanol as three different solvents to study the effect of the extracting solvents and temperature on their antioxidant, antidiabetic and antibacterial properties and total phenolic and flavonoid contents. The antioxidant properties were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assay. The α-glucosidase inhibitory assay was carried out to determine the antidiabetic potential. The antibacterial properties of the extracts were determined against four strains of bacterial species viz. , , and using Broth macro dilution method. The methanolic extracts of the plant were found to possess the highest total phenolic and total flavonoid contents. In the antioxidant assays, the cold methanolic extract was found to exhibit the highest DDPH radical scavenging activity and ferric-reducing antioxidant power. In the antidiabetic assay, the extract exhibited better α-glucosidase inhibitory potential than that of the positive control acarbose. It was also found to be effective against both gram-positive and gram-negative strains in the antibacterial assay. A flavan-type compound 4-(5,7-dimethoxychroman-2-yl)phenol was isolated from the most bioactive cold methanolic extract of the plant and characterised from its XRD, H and C NMR, HRMS and IR data.
植物源天然产物一直是药物发现中生物活性分子的丰富来源。以一种可食用药用植物 Griff. 的嫩枝为原料，分别用甲醇、水和乙醇作为三种不同溶剂进行提取，以研究提取溶剂和温度对其抗氧化、抗糖尿病和抗菌性能以及总酚和黄酮含量的影响。通过 2,2-二苯基-1-苦基肼自由基（DPPH）、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和铁还原抗氧化能力（FRAP）测定法来测定抗氧化性能。通过 α-葡萄糖苷酶抑制试验来测定抗糖尿病潜力。采用肉汤宏观稀释法测定提取物对四种细菌菌株（即 、 、 和 ）的抗菌性能。发现该植物的甲醇提取物具有最高的总酚和总黄酮含量。在抗氧化试验中，发现冷甲醇提取物表现出最高的 DPPH 自由基清除活性和铁还原抗氧化能力。在抗糖尿病试验中，该提取物表现出比阳性对照阿卡波糖更好的 α-葡萄糖苷酶抑制潜力。在抗菌试验中还发现它对革兰氏阳性菌和革兰氏阴性菌均有效。从该植物生物活性最高的冷甲醇提取物中分离出一种黄烷型化合物 4-(5,7-二甲氧基色满-2-基)苯酚，并通过 X 射线衍射、氢核磁共振、碳核磁共振、高分辨质谱和红外光谱数据对其进行了表征。
