Department of Mushroom Science, Korea National University of Agriculture and Fisheries, Jeonju, Jeonju-si, Jeollabuk-do, South Korea.
Gyeongsangnam-do Agricultural Research and Extension Services, Jinju, Republic of Korea.
PLoS One. 2024 Aug 12;19(8):e0308832. doi: 10.1371/journal.pone.0308832. eCollection 2024.
Pleurotus ostreatus is a global mushroom crop with nutritional and medicinal benefits. However, the genetic basis of several commercial traits remains unknown. To address this, we analyzed the quantitative trait loci (QTLs) for two representative cultivars, "Heuktari" and "Miso," with apparently distinct alleles. A genetic map with 11 linkage groups was constructed, in which 27 QTLs were assigned to 14 traits. The explained phenotypic variations in QTLs ranged from 7.8% to 22.0%. Relatively high LOD values of 6.190 and 5.485 were estimated for the pinheading period and the number of valid stipes, respectively. Some QTL-derived molecular markers showed potential enhancement rates of selection precision in inbred lines, especially for cap shape (50%) and cap thickness (30%). Candidate genes were inferred from the QTL regions and validated using qRT-PCR, particularly for the cysteine and glutathione pathway, in relation to cap yellowness. The molecular markers in this study are expected to facilitate the breeding of the Heuktari and Miso lines and provide probes to identify related genes in P. ostreatus.
糙皮侧耳是一种具有营养和药用价值的全球性蘑菇作物。然而，几个商业性状的遗传基础仍然未知。为了解决这个问题，我们分析了两个具有明显不同等位基因的代表性栽培品种“Heuktari”和“Miso”的数量性状基因座（QTLs）。构建了一个包含 11 个连锁群的遗传图谱，其中将 27 个 QTL 分配到 14 个性状上。QTL 解释的表型变异范围从 7.8%到 22.0%。针状期和有效菌柄数的 LOD 值分别估计为 6.190 和 5.485，相对较高。一些 QTL 衍生的分子标记显示出在自交系中提高选择精度的潜在增长率，特别是在帽形（50%）和帽厚度（30%）方面。从 QTL 区域推断候选基因，并使用 qRT-PCR 进行验证，特别是与帽部黄色有关的半胱氨酸和谷胱甘肽途径。本研究中的分子标记有望促进 Heuktari 和 Miso 品系的培育，并提供探针以鉴定糙皮侧耳中的相关基因。
