在酿酒酵母中自主复制的人类染色体DNA序列的特征分析。
Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae.
作者信息
Montiel J F, Norbury C J, Tuite M F, Dobson M J, Mills J S, Kingsman A J, Kingsman S M
出版信息
Nucleic Acids Res. 1984 Jan 25;12(2):1049-68. doi: 10.1093/nar/12.2.1049.
Abstract
We have characterised two restriction fragments, isolated from a "shotgun" collection of human DNA, which function as autonomously replicating sequences (ARSs) in Saccharomyces cerevisiae. Functional domains of these fragments have been defined by subcloning and exonuclease (BAL 31) deletion analysis. Both fragments contain two spatially distinct domains. One is essential for high frequency transformation and is termed the Replication Sequence (RS) domain, the other, termed the Replication Enhancer (RE) domain, has no inherent replication competence but is essential for ensuring maximum function of the RS domain. The nucleotide sequence of these domains reveals several conserved sequences one of which is strikingly similar to the yeast ARS consensus sequence.
摘要
我们已经鉴定了从人类DNA“鸟枪法”文库中分离出的两个限制性片段，它们在酿酒酵母中作为自主复制序列（ARS）发挥作用。这些片段的功能域已通过亚克隆和核酸外切酶（BAL 31）缺失分析来确定。两个片段都包含两个空间上不同的结构域。一个对高频转化至关重要，被称为复制序列（RS）结构域，另一个被称为复制增强子（RE）结构域，它本身没有复制能力，但对于确保RS结构域的最大功能至关重要。这些结构域的核苷酸序列揭示了几个保守序列，其中一个与酵母ARS共有序列惊人地相似。
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本文引用的文献
1
Chromosome Mapping in Saccharomyces: Centromere-Linked Genes.酿酒酵母中的染色体图谱：着丝粒连锁基因。
Genetics. 1960 Aug;45(8):1085-110. doi: 10.1093/genetics/45.8.1085.
2
Analysis of sequences conferring autonomous replication in baker's yeast.对在面包酵母中赋予自主复制能力的序列的分析。
EMBO J. 1983;2(9):1571-5. doi: 10.1002/j.1460-2075.1983.tb01626.x.
3
Mapping sequences in loops of nuclear DNA by their progressive detachment from the nuclear cage.通过将核DNA环中的序列逐步从核笼中分离来进行映射。
Nucleic Acids Res. 1980 Jul 11;8(13):2895-906. doi: 10.1093/nar/8.13.2895.
4
Origin of replication from Xenopus laevis mitochondrial DNA promotes high-frequency transformation of yeast.非洲爪蟾线粒体DNA的复制起点促进酵母的高频转化。
Proc Natl Acad Sci U S A. 1981 May;78(5):3128-32. doi: 10.1073/pnas.78.5.3128.
5
Autonomously replicating sequences in Saccharomyces cerevisiae.酿酒酵母中的自主复制序列。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6329-33. doi: 10.1073/pnas.77.11.6329.
6
Isolation of chromosomal origins of replication in yeast.酵母中染色体复制起点的分离
Nature. 1980 Mar 13;284(5752):185-7. doi: 10.1038/284185a0.
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Mol Cell Biol. 1981 Jun;1(6):535-43. doi: 10.1128/mcb.1.6.535-543.1981.
8
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10
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