Song Qiying, Li Yao, Zhou Tao, Xiao Meiqun, Xiao Bin, Wang Mengying, Zhu Yuanfang
Department of Child Healthcare, Shenzhen Baoan Women's and Children's Hospital, Shenzhen, China.
Department of Obstetrics, Shenzhen Baoan Women's and Children's Hospital, Shenzhen, China.
Front Nutr. 2024 Jul 29;11:1428356. doi: 10.3389/fnut.2024.1428356. eCollection 2024.
To prospectively explore the association of maternal serum 25(OH)D levels with the infant's gut microbiota in Chinese populations, and to evaluate its potential influence on the dynamic change patterns of offspring's gut microbiota from 1 to 6 months old.
Eighty-seven mother-infant dyads (vitamin D insufficient group vs. normal group = 59 vs. 28) were included in this longitudinal study. Two fecal samples were collected for the included infant at home by the parents at 1 month of age ("M1 phase") and 6 months of age ("M6 phase"). Gut microbiota were profiled by 16S rRNA gene sequencing. We performed mixed effects models on alpha diversity metrics, PERMANOVA tests on beta diversity distances, and linear discriminant analysis (LDA) to identify differently abundant taxa.
We observed significantly lower Pielou's evenness and Shannon diversity in the vitamin D insufficient group in the M6 phase ( = 0.049 and 0.015, respectively), but not in the M1 phase ( > 0.05), and the dynamic changes in alpha diversity from 1 to 6 months old were significantly different according to maternal vitamin D status ( < 0.05). There were also significant differences in gut microbiota composition between the vitamin D insufficient group and normal group, both in the M1 and M6 phases (LDA score > 2.0, < 0.05). Moreover, among the predicted metagenome functions, pathways related to amino acid biosynthesis, starch degradation, and purine nucleotides biosynthesis were enriched in the vitamin D insufficient group.
Our findings highlight that maternal vitamin D status plays a pivotal role in shaping the early-life gut microbiota of the next generation.
前瞻性探索中国人群中母亲血清25(OH)D水平与婴儿肠道微生物群的关联，并评估其对后代1至6个月龄肠道微生物群动态变化模式的潜在影响。
本纵向研究纳入了87对母婴二元组（维生素D不足组与正常组分别为59对和28对）。父母在家中于婴儿1月龄（“M1期”）和6月龄（“M6期”）采集两份粪便样本。通过16S rRNA基因测序对肠道微生物群进行分析。我们对α多样性指标进行混合效应模型分析，对β多样性距离进行PERMANOVA检验，并进行线性判别分析（LDA）以识别丰度不同的分类群。
我们观察到，在M6期，维生素D不足组的皮洛均匀度和香农多样性显著降低（分别为=0.049和0.015），但在M1期未出现这种情况（>0.05），并且根据母亲维生素D状态，1至6个月龄期间α多样性的动态变化存在显著差异（<0.05）。在M1期和M6期，维生素D不足组与正常组之间的肠道微生物群组成也存在显著差异（LDA评分>2.0，<0.05）。此外，在预测的宏基因组功能中，与氨基酸生物合成、淀粉降解和嘌呤核苷酸生物合成相关的途径在维生素D不足组中富集。
我们的研究结果突出表明，母亲的维生素D状态在塑造下一代早期肠道微生物群方面起着关键作用。
