Cheng Jiahua, Wu Qiqi, Sun Rui, Li Wujuan, Wang Zhuoling, Zhou Min, Yang Tian, Wang Jing, Lyu Yuhong, Yue Changwu
Yan'an Key Laboratory of Microbial Drug Innovation and Transformation, School of Basic Medicine, Yan'an University, Yan'an, China.
Clinical Laboratory, Xi'an Daxing Hospital, Xi'an, China.
Front Microbiol. 2024 Jul 29;15:1438928. doi: 10.3389/fmicb.2024.1438928. eCollection 2024.
To explore the effects of probiotic fermentation products of germinated grains on cognitive and sleep improvement in mice with sleep deprivation induced by chlorophenylalanine (PCPA), and to provide theoretical and experimental basis for the development of natural products to alleviate insomnia.
ELISA and high-performance liquid chromatography (HPLC) were used to determine the contents of γ-aminobutyric acid and L-theanine in fermentation products. Open Field Test was used to analyze the changes of emotional behavior between groups before and after intervention. ELISA was used to analyze the changes of hypothalamic serotonin, GABA, glutamate, and serum interleukin 6. 16S rRNA sequencing was used to analyze the changes of intestinal flora before and after the intervention of compound fermentation products. LC-MS/MS was used to analyze the changes of intestinal SCFAs before and after the intervention.
The content of GABA and L-theanine in 7 L fermentation products was 12.555 μmol/L (1.295 mg/L) and 0.471 mg/mL by ELISA. Compared with the PCPA-induced Model group, the sleep duration of the KEY group was statistically significant ( 0.0001). Compared with the PCPA-induced Model group, the number of crossing the central lattice in the KEY group was significantly increased, and the number of grooming was significantly reduced (all < 0.05), suggesting that the anxiety behavior of the mice was improved. In addition, this study found that the compound fermentation products could significantly increase the content of neurotransmitters such as 5-HT, GABA and Glu in the hypothalamus of mice, reduce the content of inflammatory factors such as and in serum, regulate the structure of intestinal flora and increase the content of short-chain fatty acids.
Probiotic fermentation products of germinated grains can significantly improve sleep deprivation in PCPA mice, which may be related to regulating the levels of neurotransmitters and inflammatory factors, improving the structure of intestinal flora, and increasing the content of short-chain fatty acids. This study provides new candidates and research directions for the development of natural drugs to alleviate insomnia.
探讨发芽谷物益生菌发酵产物对氯苯丙氨酸（PCPA）诱导的睡眠剥夺小鼠认知和睡眠改善的影响，为开发缓解失眠的天然产物提供理论和实验依据。
采用酶联免疫吸附测定（ELISA）和高效液相色谱法（HPLC）测定发酵产物中γ-氨基丁酸和L-茶氨酸的含量。采用旷场试验分析干预前后各组情绪行为的变化。采用ELISA分析下丘脑5-羟色胺、γ-氨基丁酸、谷氨酸以及血清白细胞介素6的变化。采用16S核糖体RNA测序分析复合发酵产物干预前后肠道菌群的变化。采用液相色谱-串联质谱法（LC-MS/MS）分析干预前后肠道短链脂肪酸的变化。
ELISA检测显示，7L发酵产物中γ-氨基丁酸和L-茶氨酸的含量分别为12.555μmol/L（1.295mg/L）和0.471mg/mL。与PCPA诱导的模型组相比，KEY组的睡眠时间具有统计学意义（P<0.0001）。与PCPA诱导的模型组相比，KEY组穿越中央格的次数显著增加，理毛次数显著减少（均P<0.05），表明小鼠的焦虑行为得到改善。此外，本研究发现复合发酵产物可显著增加小鼠下丘脑5-羟色胺、γ-氨基丁酸和谷氨酸等神经递质的含量，降低血清中白细胞介素6等炎症因子的含量，调节肠道菌群结构并增加短链脂肪酸的含量。
发芽谷物益生菌发酵产物可显著改善PCPA小鼠的睡眠剥夺，这可能与调节神经递质和炎症因子水平、改善肠道菌群结构以及增加短链脂肪酸含量有关。本研究为开发缓解失眠的天然药物提供了新的候选物和研究方向。
