Pramanik Soudipta, Sil Alok Kumar
Department of Microbiology, University of Calcutta, 35, Ballygunge Circular Road, Ballygunge, Kolkata, 700019 India.
Food Sci Biotechnol. 2024 Feb 19;33(11):2597-2610. doi: 10.1007/s10068-023-01515-7. eCollection 2024 Aug.
Existing literature documents the beneficial effects of probiotics against atherosclerosis, a major cause of human death. However, it suffers from a serious limitation due to horizontal gene transfer. Therefore, currently, efforts are targeted to examine the beneficial effects of metabolites obtained from probiotics. In this context, the current study isolated a bacterium from yogurt and investigated the effect of its metabolites on foam cell formation, a key event for developing atherosclerosis. Results showed that the cell-free conditioned medium (CM) of this isolate and di-chloro methane extract of CM (CME) not only prevented the formation but also reduced the level of preformed foam cells. To understand the mechanism, the GC-MS study revealed the presence of compounds known to exert anti-atherogenic activities like anti-oxidant, anti-NF-κB, and lipolytic activities. Consistently, CME exhibited substantial anti-oxidant and anti-NF-κB activity. In conclusion, metabolites of this bacterium have anti-atherogenic activities and thus have therapeutic potential.
The online version contains supplementary material available at 10.1007/s10068-023-01515-7.
现有文献记载了益生菌对动脉粥样硬化（人类死亡的主要原因之一）的有益作用。然而，由于水平基因转移，该领域存在严重局限性。因此，目前的研究重点是考察益生菌代谢产物的有益作用。在此背景下，本研究从酸奶中分离出一种细菌，并研究了其代谢产物对泡沫细胞形成（动脉粥样硬化发展的关键事件）的影响。结果表明，该分离株的无细胞条件培养基（CM）及其二氯甲烷提取物（CME）不仅能阻止泡沫细胞形成，还能降低已形成的泡沫细胞水平。为了解其作用机制，气相色谱 - 质谱联用（GC - MS）研究揭示了一些具有抗氧化、抗核因子κB（NF - κB）和脂解活性等抗动脉粥样硬化活性的化合物的存在。一致地，CME表现出显著的抗氧化和抗NF - κB活性。总之，该细菌的代谢产物具有抗动脉粥样硬化活性，因此具有治疗潜力。
在线版本包含可在10.1007/s10068 - 023 - 01515 - 7获取的补充材料。
