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通用冷 RNA 相变。

Universal cold RNA phase transitions.

机构信息

Small Biosystems Lab, Condensed Matter Physics Department, Universitat de Barcelona, Barcelona 08028, Spain.

Institut de Nanociència i Nanotecnologia, Universitat de Barcelona, Barcelona 08028, Spain.

出版信息

Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2408313121. doi: 10.1073/pnas.2408313121. Epub 2024 Aug 16.

DOI:10.1073/pnas.2408313121
PMID:39150781
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11348302/
Abstract

RNA's diversity of structures and functions impacts all life forms since primordia. We use calorimetric force spectroscopy to investigate RNA folding landscapes in previously unexplored low-temperature conditions. We find that Watson-Crick RNA hairpins, the most basic secondary structure elements, undergo a glass-like transition below [Formula: see text]C where the heat capacity abruptly changes and the RNA folds into a diversity of misfolded structures. We hypothesize that an altered RNA biochemistry, determined by sequence-independent ribose-water interactions, outweighs sequence-dependent base pairing. The ubiquitous ribose-water interactions lead to universal RNA phase transitions below , such as maximum stability at [Formula: see text]C where water density is maximum, and cold denaturation at [Formula: see text]C. RNA cold biochemistry may have a profound impact on RNA function and evolution.

摘要

RNA 的结构和功能多样性从原始生命形式开始就影响着所有生命形式。我们使用量热力谱技术在以前未探索的低温条件下研究 RNA 折叠景观。我们发现，沃森-克里克 RNA 发夹，最基本的二级结构元件，在 [Formula: see text]C 以下会发生玻璃态转变，此时热容突然发生变化，RNA 折叠成多种错误折叠的结构。我们假设，由序列无关的核糖-水相互作用决定的改变的 RNA 生物化学，超过了序列依赖的碱基配对。普遍存在的核糖-水相互作用导致了普遍的 RNA 相转变，例如在 [Formula: see text]C 时最大稳定性，此时水密度最大，以及在 [Formula: see text]C 时的冷变性。RNA 低温生物化学可能对 RNA 功能和进化产生深远影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/f60f4dbf2753/pnas.2408313121fig05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/bec3c1b7e6c3/pnas.2408313121fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/4ef33542d7c3/pnas.2408313121fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/9f52314a7649/pnas.2408313121fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/a8f54f60e4e5/pnas.2408313121fig04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/f60f4dbf2753/pnas.2408313121fig05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/bec3c1b7e6c3/pnas.2408313121fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/4ef33542d7c3/pnas.2408313121fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/9f52314a7649/pnas.2408313121fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/a8f54f60e4e5/pnas.2408313121fig04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7c9/11348302/f60f4dbf2753/pnas.2408313121fig05.jpg

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