He Feng, Zhang Fan, Sun Wenxian, Ning Yuese, Wang Guo-Liang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Rice (N Y). 2018 Apr 20;11(1):27. doi: 10.1186/s12284-018-0220-7.
Rice (Oryza sativa) is the main food for half of the world's population, and is considered the model for molecular biology studies of monocotyledon species. Although the rice genome was completely sequenced about 15 years ago, the function of most rice genes is still unknown.
In this study, we developed a vector toolkit that contains 42 vectors for transient expression studies in rice protoplasts and stable expression analysis in transgenic rice. These vectors have been successfully used to study protein subcellular localization, protein-protein interaction, gene overexpression, and the CRISPR/Cas9-mediated gene editing. A novel feature of these vectors is that they contain a universal multiple cloning site, which enables more than 99% of the rice coding sequences to be conveniently transferred between vectors.
The versatile vectors represent a highly efficient and high-throughput toolkit for functional analysis of rice genes.
水稻（Oryza sativa）是世界上一半人口的主要食物，被视为单子叶植物分子生物学研究的模式植物。尽管水稻基因组大约在15年前就已完成全序列测定，但大多数水稻基因的功能仍然未知。
在本研究中，我们开发了一个载体工具包，其中包含42个载体，用于水稻原生质体中的瞬时表达研究和转基因水稻中的稳定表达分析。这些载体已成功用于研究蛋白质亚细胞定位、蛋白质-蛋白质相互作用、基因过表达以及CRISPR/Cas9介导的基因编辑。这些载体的一个新特点是它们含有一个通用多克隆位点，这使得超过99%的水稻编码序列能够在载体之间方便地转移。
这些多功能载体代表了一种用于水稻基因功能分析的高效且高通量的工具包。
