Department of Mining, Metallurgical and Materials Engineering, Université Laval Faculté des Sciences et de Génie, Québec, Quebec, Canada.
Department of Microbiology and Immunology, Université Laval Faculté de Médecine, Québec, Quebec, Canada.
PLoS One. 2024 Aug 19;19(8):e0305315. doi: 10.1371/journal.pone.0305315. eCollection 2024.
The morphological dynamics of microbial cell proliferation on an antimicrobial surface at an early growth stage was studied with Escherichia coli on the surface of a gel supplied with AgNbO3 antimicrobial particles. We demonstrated an inhibitory surface concentration, analogous to minimum inhibitory concentration, beyond which the growth of colonies and formation of biofilm are inhibited. In contrast, at lower concentrations of particles, after a lag time the cells circumvent the antimicrobial activity of the particles and grow with a rate similar to the case in the absence of particles. The lag time depends on the surface concentration of the particles and amounts to 2 h at a concentration of ½ minimum inhibitory concentration. The applicability of these findings, in terms of estimating inhibitory surface concentration, was tested in the case of antimicrobial polymethyl methacrylate (PMMA) bone cement.
研究了在早期生长阶段，抗菌表面上微生物细胞增殖的形态动力学，以大肠杆菌在含有 AgNbO3 抗菌颗粒的凝胶表面上的生长为例。我们证明了一种抑制表面浓度，类似于最小抑菌浓度，超过该浓度后，菌落的生长和生物膜的形成受到抑制。相比之下，在较低浓度的颗粒下，细胞经过滞后时间后会绕过颗粒的抗菌活性，并以类似于无颗粒存在的情况下的速率生长。滞后时间取决于颗粒的表面浓度，在浓度为最低抑菌浓度的一半时，滞后时间为 2 小时。这些发现的适用性，在估计抑制表面浓度方面，在抗菌聚甲基丙烯酸甲酯（PMMA）骨水泥的情况下进行了测试。
