College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
National Forestry and Grass Bureau of Chinese Fir Engineering Technology Research Center, Fuzhou, Fujian, China.
PeerJ. 2024 Aug 16;12:e17850. doi: 10.7717/peerj.17850. eCollection 2024.
The bark of Chinese fir (), the largest afforestation tree species in the forest areas of southern China, is susceptible to injuries and bites from small animals. The population of small animals has recently increased owing to improvements in the ecological environment across various forested areas, thus increasing the incidence of injuries in the bark of Chinese fir. Following such injuries, the bark secretes light yellow or milky white secretions, the function of which remains unclear. The present study aimed to reveal the antibacterial effect of exudates of different Chinese fir cultivars on five bacterial species.
The research involved three-year-old plantations of var and Yangkou3 and three-year-old container plantations of var, Yang 061, and Yang 020. The antibacterial effects of exudates were analyzed using the filter paper diffusion method. The minimum inhibitory concentration for each secretion and the bacterial inhibition zone were determined.
The exudates of the different Chinese fir bark exhibited notable antibacterial effects on , , , , and . However, the extent of these antibacterial effects varied among the different Chinese fir cultivars, as the minimum inhibitory concentrations (MICs) of the exudates against the five bacterial species varied. The mean MIC of was lower potency, whereas that of was the lowest. Notably, the antibacterial efficacy of the exudates was mainly influenced by the composition of the secretions rather than the number of secretions, with organic acid compounds and terpenoids potentially contributing to the antibacterial effects against and , respectively.
This study demonstrates the antibacterial effect of wound secretion of different Chinese fir cultivars, highlighting their varying efficacy on different bacterial species. Moreover, the antibacterial ability of the exudates of the strains was mainly determined by the composition of the wound secretions, and there was no noticeable relationship with the number of wound secretions. The results of this study offers a theoretical basis for screen Chinese fir cultivars with high-disease-resistant.
中国冷杉（）的树皮容易受到小动物的伤害和咬伤，中国冷杉是中国南方森林地区最大的造林树种。由于各林区生态环境的改善，小动物的数量最近有所增加，从而增加了中国冷杉树皮受伤的发生率。受伤后，树皮会分泌出淡黄色或乳白色的分泌物，其功能尚不清楚。本研究旨在揭示不同中国冷杉品种分泌物对 5 种细菌的抑菌作用。
本研究涉及 3 年生林分的 var 和 Yangkou3 以及 3 年生容器林分的 var、Yang 061 和 Yang 020。采用滤纸片扩散法分析分泌物的抑菌作用，测定各分泌物的最小抑菌浓度（MIC）和抑菌圈。
不同中国冷杉树皮分泌物对 、 、 、 和 表现出明显的抑菌作用。然而，不同中国冷杉品种分泌物的抑菌效果存在差异，因为不同分泌物对 5 种细菌的最小抑菌浓度（MIC）不同。 的分泌物抑菌作用最低，而 的抑菌作用最低。值得注意的是，分泌物的抑菌效果主要受分泌物成分的影响，而不是分泌物数量的影响，有机酸化合物和萜类化合物可能分别对 和 的抑菌作用有贡献。
本研究表明，不同中国冷杉品种的伤口分泌物具有抑菌作用，其对不同细菌的抑菌效果存在差异。此外，菌株分泌物的抑菌能力主要取决于伤口分泌物的组成，与伤口分泌物的数量没有明显关系。本研究结果为筛选具有高抗病性的中国冷杉品种提供了理论依据。
