Banks K L
J Protozool. 1979 Feb;26(1):103-8. doi: 10.1111/j.1550-7408.1979.tb02740.x.
Trypanosoma congolense Broden, an intravascular parasite, binds to vessel walls and erythrocytes of infected hosts. In an attempt to characterize T. congolense adhesion to host cells, an in vitro assay was devised. It was shown in the in vitro experiments that T. congolense binds to bovine, sheep, and goat erythrocytes, but not always to erythrocytes of rats, mice, rabbits, horses or humans. Only the anterior part of live trypanosomes adheres to erythrocytes, and the attachment site on the trypanosomes is destroyed by trypsin and chymotrypsin-trypanosomes did not adhere to bovine erythrocytes that had been incubated with neuraminidase, sodium periodate and poly-L-lysine. The foregoing experiments suggest that the surface of T. congolense contains a protein-associated site which binds to sialic acid of some host cells. This surface site is most likely responsible for attachment to blood vessels in vivo.
刚果锥虫（布罗登氏锥虫）是一种血管内寄生虫，它会黏附在受感染宿主的血管壁和红细胞上。为了描述刚果锥虫与宿主细胞的黏附特性，设计了一种体外试验。体外实验表明，刚果锥虫能与牛、绵羊和山羊的红细胞结合，但并非总能与大鼠、小鼠、兔子、马或人类的红细胞结合。只有活锥虫的前部会黏附在红细胞上，并且锥虫上的黏附位点会被胰蛋白酶和糜蛋白酶破坏——锥虫不会黏附在经神经氨酸酶、高碘酸钠和聚-L-赖氨酸处理过的牛红细胞上。上述实验表明，刚果锥虫的表面含有一个与蛋白质相关的位点，该位点能与某些宿主细胞的唾液酸结合。这个表面位点很可能是其在体内黏附血管的原因。
