Rolfe M
Genetics Division, National Institute for Medical Research, London, Great Britain.
Curr Genet. 1985;9(7):533-8. doi: 10.1007/BF00381164.
Differential colony hybridisation has been used to identify DNA sequences in Saccharomyces cerevisiae corresponding to RNA transcripts whose levels increase 5-10 fold following UV-irradiation. Four sequences have been identified, three of which share sequence homology and hybridize to the same set of genomic DNA fragments. The fourth sequence appears to be distinct, however each DNA sequence hybridizes to a similar sized RNA transcript which is approximately 4.0 kb long. The relationships between these DNA sequences and their potential protein products is discussed.
差异菌落杂交已被用于鉴定酿酒酵母中与RNA转录本相对应的DNA序列,这些RNA转录本在紫外线照射后水平增加5至10倍。已鉴定出四个序列,其中三个具有序列同源性,并与同一组基因组DNA片段杂交。然而,第四个序列似乎是不同的,但是每个DNA序列都与一个大小相似的RNA转录本杂交,该转录本大约长4.0 kb。本文讨论了这些DNA序列与其潜在蛋白质产物之间的关系。