Cellulose and Paper Department, National Research Centre, 33 El-Bohouth St., Dokki, P.O. 12622 Giza, Egypt.
Water Pollution Research Department, National Research Centre, El-Bohouth St. 33, Dokki, P.O. 12622 Giza, Egypt.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134874. doi: 10.1016/j.ijbiomac.2024.134874. Epub 2024 Aug 19.
The escalating interest in bacterial cellulose (BC) confronts a substantial obstacle due to its biologically inert properties. Hence, BC was modified with ethanolic mango peel extract (EEMP) for various industrial and medical applications of the novel nanocomposite (BC/EEMP). High-performance liquid chromatography (HPLC) delineated the phenolic composition of EEMP, revealing a repertoire of polyphenolic compounds, notably chlorogenic acid, gallic acid, catechin, and ellagic acid. EEMP exhibited broad-spectrum antimicrobial activity against Candida albicans and Staphylococcus aureus, with MIC of 0.018 mg/mL and 0.009 mg/mL, respectively. The removal mechanism of Pb and Ni by BC/EEMP nanocomposite membrane via SEM, EDX, FT-IR, and XRD was characterized, indicating deposition and aggregation of heavy metals with diminished porosity. Heavy metal removal optimization using the Box-Behnken design achieved maximal removal of 95.5 % and 90 % for Pb and Ni, respectively. Moreover, BC/EEMP nanocomposite demonstrated selective dose-dependent anticancer activity toward hepatoma (HepG-2, IC of 208.8 μg/mL), skin carcinoma (A431, IC of 216.7 μg/mL), and breast carcinoma (MDA, IC of 197.5 μg/mL), attributed to the enhanced availability of biologically active polyphenolic compounds and physical characteristics of BC. This study underscores the remarkable potential of BC/EEMP nanocomposite for multifaceted industrial and biomedical applications, marking a pioneering contribution to the field.
由于细菌纤维素（BC）的生物惰性性质，人们对其兴趣日益增加，但这也构成了一个巨大的障碍。因此，我们用乙醇芒果皮提取物（EEMP）对 BC 进行了改性，以用于新型纳米复合材料（BC/EEMP）的各种工业和医疗应用。高效液相色谱（HPLC）分析了 EEMP 的酚类成分，揭示了其包含多种多酚化合物，特别是绿原酸、没食子酸、儿茶素和鞣花酸。EEMP 对白色念珠菌和金黄色葡萄球菌表现出广谱的抗菌活性，MIC 分别为 0.018 mg/mL 和 0.009 mg/mL。通过 SEM、EDX、FT-IR 和 XRD 对 BC/EEMP 纳米复合膜去除 Pb 和 Ni 的机制进行了表征，表明重金属通过沉积和聚集而去除，同时复合膜的孔隙度降低。使用 Box-Behnken 设计对重金属去除进行了优化，结果表明，BC/EEMP 纳米复合膜对 Pb 和 Ni 的去除率最高分别达到 95.5%和 90%。此外，BC/EEMP 纳米复合材料对肝癌（HepG-2，IC 为 208.8 μg/mL）、皮肤癌（A431，IC 为 216.7 μg/mL）和乳腺癌（MDA，IC 为 197.5 μg/mL）具有选择性的剂量依赖性抗癌活性，这归因于生物活性多酚化合物的增强可用性和 BC 的物理特性。这项研究突出了 BC/EEMP 纳米复合材料在多方面工业和生物医学应用中的巨大潜力，为该领域做出了开创性的贡献。
