Iosif Laura, Țâncu Ana Maria Cristina, Amza Oana Elena, Dimitriu Bogdan, Ispas Ana, Pantea Mihaela, Imre Marina
MD, PhD, Department of Prosthodontics, Faculty of Dentistry, "Carol Davila" University of Medicine and Pharmacy, 17-23 Plevnei Street, 010221 Bucharest, Romania.
MD, PhD, Department of Endodontics, Faculty of Dentistry, University of Medicine and Pharmacy "Carol Davila", 17-23 Plevnei Street, 020021 Bucharest, Romania.
Germs. 2024 Mar 31;14(1):28-37. doi: 10.18683/germs.2024.1415. eCollection 2024 Mar.
Assessment of the denture plaque can provide a valuable report regarding the oral health of geriatric patients and the oral hygiene habits in this population group. Focusing on the current research gap on this topic in Romania, our research aims to highlight the microbial plaque on the different surfaces and types of removable poly(methyl methacrylate) (PMMA) prostheses to qualitatively evaluate the microbial species, and to assess the combined effect of mechanical and chemical cleaning of dentures on the prosthetic microbiome.
The prosthetic plaque from four denture wearers was identified with a UV activated fluorescent revealer and digitally photographed. Swab samples according to a certain hygiene protocol were cultured on three different growth media such as blood agar (BA), bromothymol blue lactose agar (AABTL) and Sabouraud dextrose agar (SDA).
Denture plaque was variably distributed on the surface of the prostheses. Regardless of the denture type (conventional complete denture, tooth or implant supported overdenture, removable partial denture) the microbial plaque was identified at the retentive areas, especially at interdental spaces. The main plaque deposition areas were the vestibular incline of the labial flange of the maxillary denture and the lingual incline of the posterior lingual flange of the mandibular denture. The prosthetic microbiome consisted of pathogenic Gram-positive aerobes spp. and coagulase-negative spp.), Gram-positive anaerobes spp. and spp.) as well as subspecies of fungi of the genus .
Despite its poor variety, the denture microbiome in the investigated cases hosts colonies with high pathogenic potential. Some areas of the prostheses are more likely to accumulate dental plaque. Dentists should insist through more regular checks of the patients and their caregivers on ensuring the biocontrol of the dentures, especially in frail geriatric patients exposed to greater risks related to general health.
义齿菌斑评估可为老年患者的口腔健康及该人群的口腔卫生习惯提供有价值的报告。鉴于罗马尼亚在该主题上目前存在研究空白，我们的研究旨在突出不同表面及类型的可摘聚甲基丙烯酸甲酯（PMMA）假体上的微生物菌斑，以定性评估微生物种类，并评估义齿机械清洁和化学清洁对假体微生物群的综合影响。
用紫外线激活荧光显影剂识别四名义齿佩戴者的假体菌斑并进行数码拍照。按照特定卫生方案采集的拭子样本在三种不同的生长培养基上培养，如血琼脂（BA）、溴麝香草酚蓝乳糖琼脂（AABTL）和沙氏葡萄糖琼脂（SDA）。
义齿菌斑在假体表面分布各异。无论义齿类型（传统全口义齿、牙支持或种植支持覆盖义齿、可摘局部义齿）如何，微生物菌斑均在固位区域被识别，尤其是牙间隙处。主要的菌斑沉积区域是上颌义齿唇侧基托的前庭斜面和下颌义齿后舌侧基托的舌侧斜面。假体微生物群由致病性革兰氏阳性需氧菌（如 spp.和凝固酶阴性 spp.）、革兰氏阳性厌氧菌（如 spp.和 spp.）以及 属真菌亚种组成。
尽管种类有限，但在所调查病例中义齿微生物群含有具有高致病潜力的菌落。假体的某些区域更容易积聚牙菌斑。牙医应通过更定期地检查患者及其护理人员，坚持确保义齿的生物控制，尤其是在面临更多与总体健康相关风险的体弱老年患者中。
