Zeisbrich M, Rzepka R, Finzel S, Venhoff N, Voll R E
Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Freiburg im Breisgau, Germany.
Scand J Rheumatol. 2025 Mar;54(2):125-134. doi: 10.1080/03009742.2024.2387483. Epub 2024 Aug 22.
In systemic lupus erythematosus (SLE), the non-classical monocyte compartment is expanded, but its phenotype and association with clinical disease manifestations have not been explored.
Monocyte subsets from 39 SLE patients, 32 healthy age-matched controls, and 16 patients from a disease control (autoimmune connective tissue disease other than SLE) were determined based on CD14 and CD16 surface expression. Cell surface expression of the receptors for macrophage colony-stimulating factor (M-CSF) (CD115) and granulocyte-macrophage colony-stimulating factor (GM-CSF) (CD116), as well as 6-Sulpho LacNAc (slan), were analysed by flow cytometry. The association of monocyte populations with disease manifestations, disease activity markers, and current medication of each patient was analysed by chart review.
Non-classical monocytes displayed a cell-type specific signature of high M-CSF receptor CD115 and low GM-CSF receptor CD116 expression that separated them from the other two monocyte subsets. In healthy individuals, the M-CSF receptor on non-classical monocytes was an age-dependent surface marker, with lower expression in young adults. However, SLE monocytes were characterized by a marked expansion of M-CSF receptor/CD115 non-classical monocytes in patients of all ages. The expanded population of M-CSF receptor/CD115 non-classical monocytes was associated with lupus nephritis but not with disease activity, and coexpressed slan.
The non-classical monocyte subset in SLE is characterized by an expansion of M-CSF receptor/CD115 cells that are associated with lupus nephritis and coexpress slan.
在系统性红斑狼疮（SLE）中，非经典单核细胞区室扩大，但其表型以及与临床疾病表现的关联尚未得到研究。
根据CD14和CD16表面表达，确定了39例SLE患者、32例年龄匹配的健康对照者以及16例疾病对照者（除SLE外的自身免疫性结缔组织病患者）的单核细胞亚群。通过流式细胞术分析巨噬细胞集落刺激因子（M-CSF）受体（CD115）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）受体（CD116）以及6-硫酸乳糖胺（slan）的细胞表面表达。通过查阅病历分析单核细胞群体与每位患者的疾病表现、疾病活动标志物和当前用药之间的关联。
非经典单核细胞表现出一种细胞类型特异性特征，即高M-CSF受体CD115和低GM-CSF受体CD116表达，这使其与其他两个单核细胞亚群区分开来。在健康个体中，非经典单核细胞上的M-CSF受体是一种年龄依赖性表面标志物，在年轻人中表达较低。然而，SLE单核细胞的特征是在所有年龄段的患者中，M-CSF受体/CD115非经典单核细胞显著扩增。M-CSF受体/CD115非经典单核细胞的扩增群体与狼疮性肾炎相关，但与疾病活动无关，且共表达slan。
SLE中的非经典单核细胞亚群的特征是M-CSF受体/CD115细胞扩增，这些细胞与狼疮性肾炎相关且共表达slan。
