College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Bioresour Technol. 2024 Nov;411:131302. doi: 10.1016/j.biortech.2024.131302. Epub 2024 Aug 22.
Microbial biofilms have gained significant traction in commercial wastewater treatment due to their inherent resilience, well-organized structure, and potential for collaborative metabolic processes. As our understanding of their physiology deepens, these living catalysts are finding exciting applications beyond wastewater treatment, including the production of bulk and fine chemicals, bioelectricity generation, and enzyme immobilization. While the biological applications of biofilms in different biocatalytic systems have been extensively summarized, the applications of artificially engineered biofilms were rarely discussed. This review aims to bridge this gap by highlighting the untapped potential of engineered microbial biofilms in diverse biocatalytic applications, with a focus on strategies for biofilms engineering. Strategies for engineering biofilm-based systems will be explored, including genetic modification, synthetic biology approaches, and targeted manipulation of biofilm formation processes. Finally, the review will address key challenges and future directions in developing robust biofilm-based biocatalytic platforms for large-scale production of chemicals, pharmaceuticals, and biofuels.
微生物生物膜因其固有的弹性、良好的组织结构和潜在的协同代谢过程，在商业废水处理中得到了广泛的关注。随着我们对其生理学的深入了解，这些活催化剂在废水处理之外的领域找到了令人兴奋的应用，包括大宗和精细化学品的生产、生物电能的产生和酶的固定化。虽然生物膜在不同生物催化系统中的生物学应用已经被广泛总结，但人工设计的生物膜的应用却很少被讨论。本综述旨在通过强调工程化微生物生物膜在各种生物催化应用中的未开发潜力来弥补这一空白，重点关注生物膜工程策略。将探讨基于生物膜的系统的工程策略，包括遗传修饰、合成生物学方法以及对生物膜形成过程的靶向操纵。最后，将讨论在开发用于化学品、药物和生物燃料的大规模生产的稳健生物膜基生物催化平台方面的关键挑战和未来方向。
