Zhong Yutao, Shang Changyu, Wang Yandong, Li Jianhua, Liu Chengcai, Cui Zhiyong, Qi Qingsheng
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China.
Qiqihar Longjiang Fufeng Biotechnology Co., Ltd., Qiqihar 161031, Heilongjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2644-2665. doi: 10.13345/j.cjb.240349.
Succinic acid is an important C4 platform compound that serves as a raw material for the production of 1,4-butanediol, tetrahydrofuran, and biodegradable plastics such as polybutylene succinate (PBS). Compared to the traditional petrochemical-based route that uses maleic anhydride as a raw material, the microbial fermentation method for producing succinic acid offers more sustainable economic value and environmental friendliness. Yeasts with good acid tolerance can achieve low-pH fermentation of succinic acid, significantly reducing the cost of product extraction. Therefore, constructing high-yield succinic acid yeast strains through metabolic engineering has garnered increasing attention. This paper systematically introduced the application value and market size of succinic acid, summarized the pathways and key enzymes involved in succinic acid synthesis in microorganisms, and elaborated on the latest research progress in the synthesis of succinic acid using yeast cell factories. It also presented the current status of succinic acid synthesis using non-food raw materials such as glycerol, acetic acid, lignocellulosic hydrolysate, and others as substrates by engineered yeast strains. Finally, the paper provided a prospect for low-pH succinic acid biomanufacturing based on yeast cell factories.
琥珀酸是一种重要的C4平台化合物，用作生产1,4-丁二醇、四氢呋喃和生物可降解塑料（如聚丁二酸丁二醇酯（PBS））的原料。与以马来酸酐为原料的传统石化路线相比，微生物发酵法生产琥珀酸具有更高的可持续经济价值和环境友好性。具有良好耐酸性的酵母能够实现琥珀酸的低pH发酵，显著降低产品提取成本。因此，通过代谢工程构建高产琥珀酸酵母菌株受到越来越多的关注。本文系统介绍了琥珀酸的应用价值和市场规模，总结了微生物中琥珀酸合成的途径和关键酶，并阐述了利用酵母细胞工厂合成琥珀酸的最新研究进展。还介绍了工程酵母菌株以甘油、乙酸、木质纤维素水解产物等非食品原料为底物合成琥珀酸的现状。最后，本文对基于酵母细胞工厂的低pH琥珀酸生物制造进行了展望。
