Imaki T, Shibasaki T, Shizume K, Masuda A, Hotta M, Kiyosawa Y, Jibiki K, Demura H, Tsushima T, Ling N
J Clin Endocrinol Metab. 1985 Feb;60(2):290-3. doi: 10.1210/jcem-60-2-290.
The effect of FFA on GH-releasing hormone (GHRH)-mediated secretion of GH was examined in six normal young men. Three of the men were infused with 250 ml of a lipid-heparin solution at 1.67 ml/min for 150 min, and the other three were given an equivalent volume of saline in the same manner. Thirty minutes after the start of infusion, 100 micrograms GHRH (the 44-amino acid form) were injected iv, and plasma GH and FFA were measured. One week later, the same men participated in an identical experiment, but the ones who had received lipid-heparin previously were given saline and vice versa. In both experiments, plasma FFA increased to 2.25 +/- 0.16 meq/liter (mean +/- SEM) 60 min after the start of lipid-heparin infusion, whereas FFA levels did not change significantly in the saline-treated group. Mean plasma GH levels reached peak concentrations in both groups 30 min after GHRH treatment. However, the peak GH response when lipid-heparin was given was significantly diminished (8.4 +/- 1.7 ng/ml), compared with the peak response when saline was given (28.9 +/- 7.1 ng/ml). These data suggest that plasma FFA elevations induced by lipid-heparin infusion inhibit GH secretion induced by GHRH.
在6名正常年轻男性中研究了游离脂肪酸（FFA）对生长激素释放激素（GHRH）介导的生长激素（GH）分泌的影响。其中3名男性以1.67 ml/分钟的速度输注250 ml脂质 - 肝素溶液，持续150分钟，另外3名男性以相同方式给予等量的生理盐水。输注开始30分钟后，静脉注射100微克GHRH（44个氨基酸形式），并测量血浆GH和FFA。一周后，同样的男性参与相同的实验，但之前接受脂质 - 肝素的人给予生理盐水，反之亦然。在两个实验中，脂质 - 肝素输注开始60分钟后，血浆FFA升高至2.25±0.16 meq/升（平均值±标准误），而生理盐水处理组的FFA水平没有显著变化。两组在GHRH治疗后30分钟血浆GH水平均达到峰值浓度。然而，与给予生理盐水时的峰值反应（28.9±7.1 ng/ml）相比，给予脂质 - 肝素时的GH峰值反应显著降低（8.4±1.7 ng/ml）。这些数据表明，脂质 - 肝素输注诱导的血浆FFA升高抑制了GHRH诱导的GH分泌。
