Department of Anatomy, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Radiation Biology &Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Cardiovasc Toxicol. 2021 Jul;21(7):533-542. doi: 10.1007/s12012-021-09644-3. Epub 2021 Mar 19.
Doxorubicin (DOX) is a potent anti-cancer antibiotic that was widely used for treatment of various cancers. It produces free radicals which result in extreme dose-limiting cardiotoxicity. This study investigated the cardioprotective potential of chia seed oil, an active polyphenolic nutraceutical against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats were divided into four groups (n = 6) which consist of normal control, DOX control, test-A and test-B group. Animals were prophylactically treated with two different doses of test drug, i.e. chia seed oil 2.5 ml/kg/day and 5 ml/kg/day in test-A and test-B groups orally for 7 days. Doxorubicin (25 mg/kg; single dose) was administered intraperitoneally to DOX control, Test-A and Test-B animals on the seventh day to induce cardiotoxicity. ECG analysis was done before and after treatment. Besides ECG, CK, CK-MB, LDH, AST, MDA and GSH were analyzed. DOX had significantly altered ECG, CK, CK-MB, LDH, AST, MDA and GSH. Pre-treatment with chia seed oil significantly alleviated DOX-induced ECG changes and also guarded against DOX-induced rise of serum CK, CK-MB and AST levels. Chia seed oil alleviated histopathological alteration in DOX-treated rats. It also significantly inhibited DOX-induced GSH depletion and elevation of MDA. The present study revealed that chia seed oil exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats. Our study opens the perspective to clinical studies to precisely consider chia seed oil as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.
多柔比星（DOX）是一种有效的抗癌抗生素，广泛用于治疗各种癌症。它会产生自由基，从而导致极度剂量限制的心脏毒性。本研究旨在研究奇亚籽油的心脏保护潜力，奇亚籽油是一种具有活性的多酚类营养保健品，可预防多柔比星引起的 Wistar 大鼠心脏毒性。将 24 只雌性 Wistar 大鼠分为四组（n = 6），包括正常对照组、DOX 对照组、试验 A 组和试验 B 组。动物预防性地用两种不同剂量的试验药物治疗，即试验 A 组和试验 B 组的奇亚籽油 2.5 ml/kg/天和 5 ml/kg/天口服 7 天。第 7 天，DOX 对照组、试验 A 组和试验 B 组动物腹腔内给予多柔比星（25 mg/kg；单次剂量）以诱导心脏毒性。在治疗前后进行心电图分析。除心电图外，还分析 CK、CK-MB、LDH、AST、MDA 和 GSH。DOX 显著改变了心电图、CK、CK-MB、LDH、AST、MDA 和 GSH。奇亚籽油预处理显著缓解了 DOX 引起的心电图变化，并防止了 DOX 引起的血清 CK、CK-MB 和 AST 水平升高。奇亚籽油减轻了 DOX 处理大鼠的组织病理学改变。它还显著抑制了 DOX 诱导的 GSH 耗竭和 MDA 升高。本研究表明，奇亚籽油对雌性 Wistar 大鼠多柔比星诱导的心脏毒性具有心脏保护作用。我们的研究为临床研究开辟了视角，以准确考虑奇亚籽油作为多柔比星联合化疗中的潜在化学保护营养保健品，以限制其心脏毒性。
