Steadham J E, Stall S K, Simmank J L
Diagn Microbiol Infect Dis. 1985 Jan;3(1):33-40. doi: 10.1016/0732-8893(85)90064-1.
A total of 199 Mycobacterium tuberculosis, 182 M. kansasii, and 32 M. avium complex isolates were tested for susceptibility to either three or 10 color-coded drugs in a four-phase study using the conventional 7H10 plate method and the BACTEC system. Agreement between the two test systems for M. tuberculosis isolates against isoniazid, ethambutol, and rifampin was 96%; it was 93.6% against all 10 drugs. Disagreement was highest for ethionamide and cycloserine. M. kansasii data were encouraging, with 88.9% drug agreement against 10 drugs, but M. avium complex agreement was only 51.6% against 10. Average time required for reportable results was 4.8 days for M. tuberculosis, 4.3 days for M. kansasii, and 3.4 days for M. avium complex. Growth for 3 days in an enrichment medium yielded suspensions of the organisms in a logarithmic growth phase and reduced problems of clumps of cells in inocula.
在一项四阶段研究中，使用传统的7H10平板法和BACTEC系统，对总共199株结核分枝杆菌、182株堪萨斯分枝杆菌和32株鸟分枝杆菌复合群分离株进行了三种或十种颜色编码药物的药敏试验。两种检测系统对结核分枝杆菌分离株针对异烟肼、乙胺丁醇和利福平的一致性为96%；针对所有10种药物的一致性为93.6%。对乙硫异烟胺和环丝氨酸的分歧最大。堪萨斯分枝杆菌的数据令人鼓舞，针对10种药物的药敏一致性为88.9%，但鸟分枝杆菌复合群针对10种药物的一致性仅为51.6%。结核分枝杆菌可报告结果所需的平均时间为4.8天，堪萨斯分枝杆菌为4.3天，鸟分枝杆菌复合群为3.4天。在增菌培养基中培养3天可使细菌处于对数生长期，减少接种物中细胞团块的问题。
