Yang Lijie, Yao Bingqian, Zhang Shimin, Yang Yuting, Pan Hongbin, Zeng Xiangfang, Qiao Shiyan
Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Animal Sciences and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Anim Physiol Anim Nutr (Berl). 2025 Mar;109(2):233-242. doi: 10.1111/jpn.14023. Epub 2024 Aug 24.
In order to investigate the regularity of fecal microorganisms changes in Landrace × Large White × Duroc (DLY) and Diqing Tibetan pigs (TP) induced by dietary fiber, and further explore the buffering effect of different intestinal flora structures on dietary stress. DLY (n = 15) and TP (n = 15) were divided into two treatments. Then, diet with 20% neutral detergent fiber (NDF) was supplemented for 9 days. Our results showed that the feed conversion efficiency of TP was significantly higher (p < 0.05) than that of DLY. The fecal microorganisms shared by the two groups gradually increased with the feeding cycle. In addition, the dispersion of Shannon, Simpson, ACE and Chao of TP decreased. Also, we found that the fecal microorganisms of TP (R = 0.2089, p < 0.01) and DLY (R = 0.3982, p < 0.01) showed significant differences in different feeding cycles. With the prolongation of feeding cycle, the similarity of fecal microbial composition between DLY and TP increased. Our study strongly suggests that the complex environment and diet structure have shaped the unique gut microbiota of TP, which plays a vital role in the buffering effect of high-fiber diets.
为了探究膳食纤维对长白×大白×杜洛克（DLY）和迪庆藏猪（TP）粪便微生物变化规律的影响，并进一步探讨不同肠道菌群结构对日粮应激的缓冲作用。将15头DLY猪和15头TP猪分为两个处理组。然后，补充含20%中性洗涤纤维（NDF）的日粮，持续9天。结果表明，TP猪的饲料转化效率显著高于DLY猪（p＜0.05）。两组共有的粪便微生物随饲养周期逐渐增加。此外，TP猪的香农指数、辛普森指数、ACE指数和Chao指数的离散度降低。同时，我们发现TP猪（R = 0.2089，p＜0.01）和DLY猪（R = 0.3982，p＜0.01）在不同饲养周期的粪便微生物存在显著差异。随着饲养周期的延长，DLY猪和TP猪粪便微生物组成的相似度增加。我们的研究强烈表明，复杂的环境和日粮结构塑造了TP猪独特的肠道微生物群，其在高纤维日粮的缓冲作用中起着至关重要的作用。
