Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan.
Department of Rehabilitation Medicine, Nara Medical University, Kashihara, Nara, Japan.
Sci Rep. 2024 Aug 25;14(1):19725. doi: 10.1038/s41598-024-70512-5.
Allogeneic bone grafts are used to treat bone defects in orthopedic surgery, but the osteogenic potential of artificial bones remains a challenge. In this study, we developed a β-tricalcium phosphate (β-TCP) formulation containing MgO, ZnO, SrO, and SiO and compared its bone-forming ability with that of β-TCP without biological elements. We prepared β-TCP discs with 60% porosity containing 1.0 wt% of these biological elements. β-TCP scaffolds were loaded with bone marrow-derived mesenchymal stem cells (BMSC) from 7-week-old male rats and cultured for 2 weeks. ALP activity and mRNA expression of osteogenic markers were evaluated. In addition, scaffolds were implanted subcutaneously in rats and analyzed after 7 weeks. In vitro, the MgO group showed lower Ca concentrations and higher osteogenic marker expression compared to controls. In vivo, the MgO group showed higher ALP activity compared to controls, and RT-qPCR analysis showed significant expression of BMP2 and VEGF. Histopathology, fluorescent immunostaining, and micro-CT also showed relatively better bone formation in the MgO group. β-TCP with MgO may enhance bone morphology in vitro and in vivo and improve the prognosis of patients with substantial and refractory bone defects.
同种异体骨移植用于治疗骨科手术中的骨缺损，但人工骨的成骨潜力仍然是一个挑战。在这项研究中，我们开发了一种含有 MgO、ZnO、SrO 和 SiO 的β-磷酸三钙（β-TCP）配方，并将其成骨能力与不含生物元素的β-TCP 进行了比较。我们制备了含有 60%孔隙率和 1.0wt%这些生物元素的β-TCP 圆盘。β-TCP 支架负载了来自 7 周龄雄性大鼠的骨髓间充质干细胞（BMSC）并培养了 2 周。评估了碱性磷酸酶（ALP）活性和成骨标志物的 mRNA 表达。此外，将支架植入大鼠皮下，7 周后进行分析。体外，与对照组相比，MgO 组的 Ca 浓度较低，成骨标志物表达较高。体内，MgO 组的 ALP 活性高于对照组，实时定量 PCR（RT-qPCR）分析显示 BMP2 和 VEGF 的表达显著。组织病理学、荧光免疫染色和 micro-CT 也显示 MgO 组的骨形成相对较好。含 MgO 的β-TCP 可能会增强体外和体内的骨形态，并改善有大量和难治性骨缺损的患者的预后。
