Beydoun Safa, Sridhar Aditya, Bhandari Mira, Kitto Elizabeth S, Leiser Scott F
Molecular and Integrative Physiology, University of Michigan-Ann Arbor, Ann Arbor, Michigan, United States.
Molecular, Cellular and Developmental Biology, University of Michigan-Ann Arbor, Ann Arbor, Michigan, United States.
MicroPubl Biol. 2024 Aug 8;2024. doi: 10.17912/micropub.biology.001288. eCollection 2024.
studies can be constrained by worms escaping standard solid nematode growth medium (NGM) plates. When worms are in search of food or are avoiding pathogens, chemicals, and environmental stressors, they often exhibit a behavior known as "fleeing". Palmitic acid (PA) is sometimes used as a barrier "fence" to reduce fleeing under limited food and oxygen conditions. Here, we evaluate the efficacy of palmitic acid, polyethylene glycol (PEG) and copper as potential barriers to reduce fleeing under various environmental conditions. Our results indicate that PA and PEG each reduce fasted flee rate and do not obviously alter overall health and lifespan of the worms, while copper blunts worm growth and development. We also find that PEG is a more optimal tool than PA since it is more effective in fasted conditions, reduces flee rate in a pathogenic environment, and does not alter worm size.
研究可能会受到线虫逃离标准固体线虫生长培养基（NGM）平板的影响。当线虫寻找食物或躲避病原体、化学物质和环境应激源时，它们常常表现出一种被称为“逃离”的行为。在食物和氧气有限的条件下，有时会使用棕榈酸（PA）作为屏障“围栏”来减少线虫的逃离。在此，我们评估了棕榈酸、聚乙二醇（PEG）和铜作为潜在屏障在各种环境条件下减少线虫逃离的效果。我们的结果表明，PA和PEG均可降低禁食时的逃离率，且不会明显改变线虫的整体健康状况和寿命，而铜会抑制线虫的生长和发育。我们还发现，PEG比PA是更理想的工具，因为它在禁食条件下更有效，在致病环境中可降低逃离率，并且不会改变线虫的大小。
