Puspitasari Heni, Suwanti Lucia Tri, Mufasirin Mufasirin, Kusnoto Kusnoto, Yudaniayanti Ira Sari, Setiawan Boedi, Suprihati Endang, Aksono Eduardus Bimo, Widodo Dwi Priyo, Wardhana April Hari, Matsubayashi Makoto, Indasari Elly Nur
Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Toxoplasma Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia.
Vet World. 2024 Jul;17(7):1575-1580. doi: 10.14202/vetworld.2024.1575-1580. Epub 2024 Jul 24.
Toxoplasmosis is caused by the parasite . Cats are the only known hosts that excrete resistant oocysts. Wild rats serve as crucial reservoirs and intermediate hosts for survival and dissemination. Consuming soil and water containing oocysts can lead to illness. This study aimed to estimate the prevalence of toxoplasmosis in wild rats through molecular detection as an indicator of environmental contamination in Surabaya.
One hundred rats were collected from the three areas (housing, dense settlements, and traditional markets) and distributed into the five zones: West, East, Central, North, and South of Surabaya. Brain tissue samples were extracted using a Geneaid™ (New Taipei City, Taiwan) DNA isolation kit and analyzed through the loop-mediated isothermal amplification (LAMP) method.
The study analyzed brain tissue from 100 wild rats, consisting of 77 and 33 , displaying 30% LAMP positivity. The study revealed that 30% (30/100) of wild rats tested were infected with . The molecular prevalence rate in male rats was 32.35% (22/68), compared to females with 25% (8/32). 41.9% of the housing population, 33.3% of traditional markets, and 22.6% of dense settlements had the highest molecular prevalence. The high positive molecular rate at the trapping site can be attributed to cats and dense populations.
Thirty percentage wild rats were tested positive for toxoplasmosis in Surabaya, East Java, Indonesia using LAMP method. Implementing strict control and monitoring is crucial in preventing the transmission of diseases from wild rats to humans. It is necessary to carry out further research related to genetic analysis of to determine the type of that infects animals and humans in Surabaya through bioassay and molecular test.
弓形虫病由寄生虫引起。猫是已知唯一能排出具有抵抗力的卵囊的宿主。野生大鼠是其生存和传播的关键储存宿主和中间宿主。摄入含有卵囊的土壤和水会导致患病。本研究旨在通过分子检测估计野生大鼠中弓形虫病的流行率，以此作为泗水环境污染的指标。
从三个区域（房屋、密集居住区和传统市场）收集了100只大鼠，并分布到泗水的五个区域：西部、东部、中部、北部和南部。使用Geneaid™（中国台湾新北市）DNA提取试剂盒提取脑组织样本，并通过环介导等温扩增（LAMP）方法进行分析。
该研究分析了100只野生大鼠的脑组织，其中77只[此处原文似乎缺失部分信息]和33只[此处原文似乎缺失部分信息]，LAMP阳性率为30%。研究表明，所检测的野生大鼠中有30%（30/100）感染了[此处原文似乎缺失寄生虫名称]。雄性大鼠的分子流行率为32.35%（22/68），而雌性大鼠为25%（8/32）。房屋区域的分子流行率最高，为41.9%，传统市场为33.3%，密集居住区为22.6%。诱捕地点的高阳性分子率可归因于猫和密集的种群。
在印度尼西亚爪哇岛东部的泗水，使用LAMP方法检测出30%的野生大鼠弓形虫病呈阳性。实施严格的控制和监测对于预防疾病从野生大鼠传播给人类至关重要。有必要开展与[此处原文似乎缺失寄生虫名称]基因分析相关的进一步研究，以通过生物测定和分子检测确定在泗水感染动物和人类的[此处原文似乎缺失寄生虫名称]类型。
