鉴定抗病毒先天免疫中的 RNA 传感器。

Identifying RNA Sensors in Antiviral Innate Immunity.

机构信息

Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.

Changzhou Key Laboratory of Molecular Diagnostics and Precision Cancer Medicine, Changzhou Wujin People's Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, China.

出版信息

Methods Mol Biol. 2025;2854:107-115. doi: 10.1007/978-1-0716-4108-8_12.

DOI:10.1007/978-1-0716-4108-8_12

PMID:39192123

Abstract

The innate immune system plays a pivotal role in pathogen recognition and the initiation of innate immune responses through its Pathogen Recognition Receptors (PRRs), which detect Pathogen-Associated Molecular Patterns (PAMPs). Nucleic acids, including RNA and DNA, are recognized as particularly significant PAMPs, especially in the context of viral pathogens. During RNA virus infections, specific sequences in the viral RNA mark it as non-self, enabling host recognition through interactions with RNA sensors, thereby triggering innate immunity. Given that some of the most lethal viruses are RNA viruses, they pose a severe threat to human and animal health. Therefore, understanding the immunobiology of RNA PRRs is crucial for controlling pathogen infections, particularly RNA virus infections. In this chapter, we will introduce a "pull-down" method for identifying RIG-I-like receptors, related RNA helicases, Toll-like receptors, and other RNA sensors.

摘要

天然免疫系统通过其病原体识别受体（PRRs）在病原体识别和启动固有免疫反应中发挥关键作用，这些受体识别病原体相关分子模式（PAMPs）。核酸，包括 RNA 和 DNA，被认为是特别重要的 PAMPs，特别是在病毒病原体的情况下。在 RNA 病毒感染期间，病毒 RNA 中的特定序列将其标记为非自身，通过与 RNA 传感器的相互作用使宿主能够识别，从而触发固有免疫。鉴于一些最致命的病毒是 RNA 病毒，它们对人类和动物健康构成严重威胁。因此，了解 RNA PRRs 的免疫生物学对于控制病原体感染，特别是 RNA 病毒感染至关重要。在本章中，我们将介绍一种“下拉”方法，用于鉴定 RIG-I 样受体、相关 RNA 解旋酶、 Toll 样受体和其他 RNA 传感器。