Oh Hanseul, Kim Egan, Lee Sukjoon, Park Soyeon, Chen Dongzi, Shin Su-Jung, Kim Euiseong, Kim Sunil
Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea.
BK21 FOUR Project, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea.
Materials (Basel). 2020 Nov 20;13(22):5242. doi: 10.3390/ma13225242.
The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars ( = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.
本研究旨在从人牙周膜干细胞（hPDLSCs）的细胞活力、炎症反应、间充质表型表达、成骨潜能、细胞黏附及形态等方面，评估硅酸钙类封闭剂（CeraSeal和EndoSeal TCS）及环氧树脂类封闭剂（AH-Plus）的生物相容性。hPDLSCs取自4名年龄在16至24岁之间受试者的前磨牙（n = 4）。流式细胞术分析表明，所有材料中hPDLSCs的干性均得以维持。在细胞活力测试中，AH-Plus的细胞活力最低，而CeraSeal的细胞活力显著高于其他材料。在酶联免疫吸附测定（ELISA）测试中，AH-Plus的白细胞介素-6（IL-6）和白细胞介素-8（IL-8）表达高于硅酸钙类封闭剂。在成骨潜能测试中，AH-Plus的表达水平低于其他材料；然而，EndoSeal TCS的表达水平优于其他材料。每个细胞系的所有实验均至少重复三次。扫描电子显微镜研究显示，AH-Plus上细胞增殖程度低，而硅酸钙类封闭剂上细胞增殖程度高。在本研究中，硅酸钙类封闭剂似乎比环氧树脂类封闭剂具有更高的生物相容性和更低的细胞毒性。
