Xu Yipeng, Chen Yolanda H, Yu Xiaoping
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 310018, People's Republic of China,
In Vitro Cell Dev Biol Anim. 2014;50(5):384-8. doi: 10.1007/s11626-013-9728-8. Epub 2014 Jan 8.
The rice brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most destructive insect pests of rice in Asia. Although resistant rice varieties can be effective in managing planthopper populations, BPH has repeatedly been able to overcome resistant rice varieties. It is possible that BPH adaptation to resistant rice varieties may be related to its endosymbionts. We investigated methods for culturing BPH cells in order to study in-depth interactions between rice, BPH, and its endosymbionts. In this study, we tested EX-CELL™ 405, EX-CELL™ 420, Mitsuhashi and Maramorosch's medium, and Kimura's medium, for in vitro culture of BPH cells. Only Kimura's medium was found to be suitable for BPH cell culture, and BPH embryos around blastokinetic stage were the best source for BPH cell culture. Cells from BPH embryonic tissues adhered to the plate substrate, and the migration of cells was observed within 24 h in primary culture. After 3 mo of subculture, various types of BPH cells were successfully maintained in the Kimura's medium.
稻褐飞虱（BPH），褐飞虱（Nilaparvata lugens (Stål)），是亚洲最具破坏性的水稻害虫之一。尽管抗性水稻品种在控制飞虱种群方面可能有效，但褐飞虱一再能够克服抗性水稻品种。褐飞虱对抗性水稻品种的适应性可能与其内共生菌有关。我们研究了培养褐飞虱细胞的方法，以便深入研究水稻、褐飞虱及其内共生菌之间的相互作用。在本研究中，我们测试了EX-CELL™ 405、EX-CELL™ 420、三桥和马拉莫罗什培养基以及木村培养基用于褐飞虱细胞的体外培养。结果发现只有木村培养基适合褐飞虱细胞培养，而处于胚动期左右的褐飞虱胚胎是褐飞虱细胞培养的最佳来源。来自褐飞虱胚胎组织的细胞附着在平板底物上，在原代培养中24小时内观察到细胞迁移。经过3个月的传代培养，各种类型的褐飞虱细胞在木村培养基中成功维持。
