分离、全基因组测序和 Burkholderia 次生代谢产物的计算机基因组挖掘。
Isolation, complete genome sequencing and in silico genome mining of Burkholderia for secondary metabolites.
机构信息
Helmholtz International Lab for Anti-Infectives, State Key Laboratory of Microbial Technology, Shandong University-Helmholtz Institute of Biotechnology, Shandong University, Qingdao, 266237, People's Republic of China.
Department of Microbiology, Rajshahi Institute of Biosciences (RIB), Affi. University of Rajshahi, Rajshahi, 6212, Bangladesh.
出版信息
BMC Microbiol. 2022 Dec 30;22(1):323. doi: 10.1186/s12866-022-02692-x.
Recent years, Burkholderia species have emerged as a new source of natural products (NPs) with increasing attractions. Genome mining suggests the Burkholderia genomes include many natural product biosynthetic gene clusters (BGCs) which are new targets for drug discovery. In order to collect more Burkholderia, here, a strain S-53 was isolated from the soil samples on a mountain area in Changde, P.R. China and verified by comparative genetic analysis to belong to Burkholderia. The complete genome of Burkholderia strain S-53 is 8.2 Mbps in size with an average G + C content of 66.35%. Its taxonomy was both characterized by 16S rRNA- and whole genome-based phylogenetic trees. Bioinformatic prediction in silico revealed it has a total of 15 NP BGCs, some of which may encode unknown products. It is expectable that availability of these BGCs will speed up the identification of new secondary metabolites from Burkholderia and help us understand how sophisticated BGC regulation works.
近年来，伯克霍尔德氏菌已成为具有越来越大吸引力的天然产物（NPs）的新来源。基因组挖掘表明，伯克霍尔德氏菌基因组包含许多天然产物生物合成基因簇（BGCs），这是药物发现的新目标。为了收集更多的伯克霍尔德氏菌，本研究从中国常德山区的土壤样本中分离到一株 S-53 菌株，并通过比较遗传分析证实其属于伯克霍尔德氏菌。伯克霍尔德氏菌 S-53 菌株的全基因组大小为 8.2 Mbps，平均 G+C 含量为 66.35%。其分类学特征同时体现在 16S rRNA 和全基因组系统发育树上。生物信息学预测表明，该菌株共含有 15 个 NP BGCs，其中一些可能编码未知产物。预计这些 BGCs 的存在将加快从伯克霍尔德氏菌中鉴定新的次生代谢产物，并帮助我们了解复杂的 BGC 调控机制。
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