Yang Fang, Liu Fei-Yi, Zhong Yi-Ming
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China.
Trop Med Infect Dis. 2024 Aug 16;9(8):180. doi: 10.3390/tropicalmed9080180.
is an opportunistic pathogen often misidentified as , leading to misdiagnoses and inappropriate treatment in clinical settings. The genetic and molecular characteristics of clinically isolated remain largely unexplored. We aim to fill this knowledge gap by examining the genomic properties of and evolutionary relationships between clinical isolates of . The genomic data of 70 strains were analyzed using whole-genome sequencing. A phylogenetic tree was generated based on the gene sequences from these strains and public databases. Among the strains, the drug resistance genes with the highest carrying rates were beta-lactamase and aminoglycoside. Locally isolated strains had a higher detection rate for virulence genes than those in public databases, with yersiniabactin genes being the most prevalent. The K locus types and MLST subtypes of the strains exhibited a dispersed distribution, with O3/O3a being the predominant subtype within the O category. In total, 28 isolates carried both IncFIB(K)_Kpn3 and IncFII_pKP91 replicons. This study underscores the importance of developing more effective diagnostic tools and therapeutic strategies for infections. The continued surveillance and monitoring of strains is essential for understanding the epidemiology of infections and informing public health strategies.
是一种机会致病菌，常被误诊为 ，导致临床环境中的误诊和不适当治疗。临床分离株的遗传和分子特征在很大程度上仍未得到探索。我们旨在通过研究临床分离株的基因组特性以及它们之间的进化关系来填补这一知识空白。使用全基因组测序分析了70株 菌株的基因组数据。基于这些菌株和公共数据库的基因序列生成了系统发育树。在这些菌株中，携带率最高的耐药基因是β-内酰胺酶和氨基糖苷类。本地分离株的毒力基因检测率高于公共数据库中的菌株，耶尔森菌素基因最为普遍。这些菌株的K位点类型和多位点序列分型（MLST）亚型呈现分散分布，O3/O3a是O类别中的主要亚型。共有28株分离株同时携带IncFIB(K)_Kpn3和IncFII_pKP91复制子。本研究强调了开发针对 感染的更有效诊断工具和治疗策略的重要性。持续监测 菌株对于了解感染的流行病学情况和为公共卫生策略提供信息至关重要。
