Pang Xiaotong, Wei Xin, Wu Yanyan, Nan Shanshan, Feng Jiaqi, Wang Fang, Yao Min, Nie Cunxi
College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
School of Medicine, Shihezi University, Shihezi 832000, China.
Antioxidants (Basel). 2024 Aug 2;13(8):942. doi: 10.3390/antiox13080942.
We aimed to investigate the role of capsaicin (CAP) in modulating lipopolysaccharide (LPS)-induced hepatic and intestinal inflammation, oxidative stress, and its colonic microflora in mice. Thirty healthy male Kunming mice with similar body weights were randomly assigned to three groups: the control group (CON), the LPS group, and the CAP group, with ten mice in each group. The CON and the LPS groups received a daily dose of normal saline, respectively, while the CAP group received an equivalent dose of CAP. On the 28th day of the experiment, the LPS and the CAP groups were intraperitoneally injected with LPS, while the CON group was injected with an equal volume of normal saline. The results lead to the following conclusions. Compared to the LPS group, CAP improved the loss of hepatic lobular structure and significantly increased the duodenal villus length and ratio of villus length to crypt depth. CAP increased hepatic and colon interleukin-10 (IL-10) and decreased IL-6, IL-1β, and tumor necrosis factor (TNF-α) levels. CAP also increased hepatic catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) expression, and decreased malondialdehyde (MDA) levels. CAP significantly increased the relative abundances of , , , , , and , some of which were closely related to hepatic and colonic immune and oxidative markers. CAP also decreased the overall content of short-chain fatty acids, except for propionic acid. Overall, CAP can regulate the colon microbiota and exert anti-inflammatory and antioxidant effects. Whether CAP exerts its anti-inflammatory and antioxidant effects by modulating the colonic microflora, mainly spp. and spp., requires further investigation.
我们旨在研究辣椒素（CAP）在调节脂多糖（LPS）诱导的小鼠肝脏和肠道炎症、氧化应激及其结肠微生物群中的作用。将30只体重相近的健康雄性昆明小鼠随机分为三组：对照组（CON）、LPS组和CAP组，每组10只。CON组和LPS组分别每日给予生理盐水，而CAP组给予等量的CAP。在实验的第28天，LPS组和CAP组腹腔注射LPS，而CON组注射等量的生理盐水。结果得出以下结论。与LPS组相比，CAP改善了肝小叶结构的损伤，并显著增加了十二指肠绒毛长度以及绒毛长度与隐窝深度的比值。CAP增加了肝脏和结肠中白细胞介素-10（IL-10）的含量，并降低了IL-6、IL-1β和肿瘤坏死因子（TNF-α）的水平。CAP还增加了肝脏中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）的表达，并降低了丙二醛（MDA）的水平。CAP显著增加了 、 、 、 、 和 的相对丰度，其中一些与肝脏和结肠的免疫及氧化标记物密切相关。CAP还降低了除丙酸外的短链脂肪酸的总体含量。总体而言，CAP可以调节结肠微生物群，并发挥抗炎和抗氧化作用。CAP是否通过调节结肠微生物群（主要是 属和 属）发挥其抗炎和抗氧化作用，尚需进一步研究。
