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成纤维细胞生长因子-2与碳酸磷灰石颗粒对牙周愈合的联合作用：一项体内和体外研究

Combined Effects of Fibroblast Growth Factor-2 and Carbonate Apatite Granules on Periodontal Healing: An In Vivo and In Vitro Study.

作者信息

Miyata Naoki, Mori Shinta, Murakami Tasuku, Bizenjima Takahiro, Seshima Fumi, Imamura Kentaro, Saito Atsushi

机构信息

Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.

Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.

出版信息

Biomedicines. 2024 Jul 25;12(8):1664. doi: 10.3390/biomedicines12081664.

DOI:10.3390/biomedicines12081664
PMID:39200129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11352071/
Abstract

The aim of this study was to investigate in vivo and in vitro the effectiveness of the use of fibroblast growth factor (FGF)-2 with carbonate apatite (COAp) on periodontal healing. Periodontal defects created in the maxillary first molars in rats were treated with FGF-2, COAp, FGF-2 + COAp or left unfilled. Healing was evaluated using microcomputed tomography, histological, and immunohistochemical analyses. In vitro experiments were performed to assess cellular behaviors and the expression of osteoblastic differentiation markers in MC3T3-E1 cells. At 4 weeks, the bone volume fraction in the FGF-2 + COAp group was significantly greater than that in the COAp group, but there was no significant difference from the FGF-2 group. The FGF-2 + COAp group demonstrated greater new bone compared with the FGF-2 or COAp group. The FGF-2 + COAp group showed greater levels of osteocalcin-positive cells compared with the COAp group, but there was no significant difference from the FGF-2 group. In vitro, the FGF-2 + COAp group exhibited a greater extent of cell attachment and more elongated cells compared with the COAp group. Compared with the COAp group, the FGF-2 + COAp group showed significantly higher viability/proliferation, but the expressions of and were reduced. The results indicated that the use of FGF-2 with COAp enhanced healing in the periodontal defects. FGF-2 promoted cell attachment to and proliferation on COAp and regulated osteoblastic differentiation, thereby contributing to novel bone formation.

摘要

本研究旨在体内和体外研究成纤维细胞生长因子（FGF）-2与碳酸磷灰石（COAp）联合使用对牙周愈合的有效性。在大鼠上颌第一磨牙制造牙周缺损，分别用FGF-2、COAp、FGF-2 + COAp处理或不填充。使用微型计算机断层扫描、组织学和免疫组织化学分析评估愈合情况。进行体外实验以评估MC3T3-E1细胞的细胞行为和成骨细胞分化标志物的表达。4周时，FGF-2 + COAp组的骨体积分数显著高于COAp组，但与FGF-2组无显著差异。与FGF-2组或COAp组相比，FGF-2 + COAp组显示出更多的新骨。与COAp组相比，FGF-2 + COAp组显示出更高水平的骨钙素阳性细胞，但与FGF-2组无显著差异。在体外，与COAp组相比，FGF-2 + COAp组表现出更大程度的细胞附着和更多伸长的细胞。与COAp组相比，FGF-2 + COAp组显示出显著更高的活力/增殖，但 和 的表达降低。结果表明，FGF-2与COAp联合使用可促进牙周缺损的愈合。FGF-2促进细胞在COAp上的附着和增殖，并调节成骨细胞分化，从而有助于新骨形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1661/11352071/3437da970b17/biomedicines-12-01664-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1661/11352071/3437da970b17/biomedicines-12-01664-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1661/11352071/6e85fbed3047/biomedicines-12-01664-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1661/11352071/b57d9fb39b30/biomedicines-12-01664-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1661/11352071/3780b8bfef49/biomedicines-12-01664-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1661/11352071/3437da970b17/biomedicines-12-01664-g007.jpg

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