用于检测猪肉和猪肝中阿扎哌隆的胶体金免疫层析试纸条检测法

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver.

作者信息

Lin Lu, Wu Xiaoling, Cui Gang, Song Shanshan, Kuang Hua, Xu Chuanlai

机构信息

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

YanCheng Teachers University, Yancheng 224100, People's Republic of China.

出版信息

ACS Omega. 2020 Jan 13;5(3):1346-1351. doi: 10.1021/acsomega.9b01841. eCollection 2020 Jan 28.

In this study, a highly sensitive and specific monoclonal antibody (mAb) against azaperone was produced. The mAb belonged to the IgG2b subtype and its half maximal inhibitory concentration (IC) was 0.46 ng/mL. The limit of detection value was 0.081 ng/mL and the linear range (IC-IC) was 0.165-1.29 ng/mL. Based on this anti-azaperone mAb, a colloidal gold immunochromatographic strip assay method was established for the detection of azaperone in pork and pork liver samples. For pork samples, the cutoff value of the colloidal gold immunochromatographic strip assay was 10 ng/g, and the cutoff value was also 10 ng/g in pig liver samples. This colloidal gold immunochromatographic strip assay is a rapid, simple, and practical method for the detection of azaperone in pork and pork liver samples available in markets.

在本研究中，制备了一种针对阿扎哌隆的高灵敏度和特异性单克隆抗体（mAb）。该单克隆抗体属于IgG2b亚型，其半数最大抑制浓度（IC）为0.46 ng/mL。检测限为0.081 ng/mL，线性范围（IC-IC）为0.165-1.29 ng/mL。基于这种抗阿扎哌隆单克隆抗体，建立了一种胶体金免疫层析试纸条检测方法，用于检测猪肉和猪肝样品中的阿扎哌隆。对于猪肉样品，胶体金免疫层析试纸条检测的临界值为10 ng/g，猪肝样品中的临界值也为10 ng/g。这种胶体金免疫层析试纸条检测方法是一种快速、简便且实用的方法，可用于检测市售猪肉和猪肝样品中的阿扎哌隆。