College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Key Laboratory of Sustainable Utilization of Tropical Biological Resources of Hainan Province, School of Tropical Crops, Hainan University, Haikou 570228, China.
Genes (Basel). 2022 Feb 10;13(2):331. doi: 10.3390/genes13020331.
Plant leaf patterns and shapes are spectacularly diverse. Changing the complexity of leaflet numbers is a valuable approach to increase its nutrition and photosynthesis. Alfalfa () is the most important forage legume species and has diversified compound leaf patterns, which makes it a model species for studying compound leaf development. However, transcriptomic information from alfalfa remains limited. In this study, RNA-Seq technology was used to identify 3746 differentially expressed genes (DEGs) between multifoliate and trifoliate alfalfa. Through an analysis of annotation information and expression data, , one of the key regulators in modifiable plant development and abiotic stress response, was further analyzed. Here, thirty genes were obtained from the alfalfa genome, of which 16 had the putative miR156 binding site. A tissue expression pattern analysis showed that the miR156-targeted were divided into two classes, namely, either tissue-specific or widely expressed in all tissues. All miR156-targeted s strongly showed diversification and positive roles under drought and salt conditions. Importantly, miR156/MsSPL08 was significantly suppressed in multifoliate alfalfa. Furthermore, in the paralogous mutant of isolated from the phenotypes of mutant plants reveal that is involved not only involved the branches but also especially regulates the number of leaflets. The legume is a typical compound leaf plant; the ratio of the leaflet often affects the quality of the forage. This study sheds light on new functions of genes that regulate leaflet number development.
植物叶片的形态和形状变化万千。改变小叶数量的复杂性是增加其营养和光合作用的一种有价值的方法。紫花苜蓿是最重要的饲料豆科植物，具有多样化的复叶模式，使其成为研究复叶发育的模式物种。然而，紫花苜蓿的转录组信息仍然有限。在这项研究中，使用 RNA-Seq 技术鉴定了多小叶和三叶紫花苜蓿之间的 3746 个差异表达基因 (DEGs)。通过注释信息和表达数据分析，进一步分析了一个在可修饰植物发育和非生物胁迫反应中的关键调节因子，。在这里，从紫花苜蓿基因组中获得了三十个基因，其中 16 个具有假定的 miR156 结合位点。组织表达模式分析表明，miR156 靶向的基因分为两类，即组织特异性或在所有组织中广泛表达。所有 miR156 靶向的基因在干旱和盐胁迫条件下都表现出强烈的多样化和积极作用。重要的是，miR156/MsSPL08 在多小叶紫花苜蓿中显著受抑制。此外，在突变体中分离的基因的同源突变体的表型揭示了不仅参与分枝，而且特别调节小叶的数量。豆科植物是典型的复叶植物；小叶的比例通常会影响饲料的质量。这项研究揭示了调节小叶数量发育的基因的新功能。
