Facultad de Ingeniería, División de Investigación y Posgrado, Centro Universitario Cerro de las Campanas, Universidad Autónoma de Querétaro, Querétaro, Qro. C.P. 76010, Mexico.
Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Pedro Escobedo, Qro. C.P. 76703, Mexico.
Int J Mol Sci. 2024 Aug 19;25(16):8996. doi: 10.3390/ijms25168996.
In this study, the biosynthesis of polyhydroxyalkanoates (PHAs) was carried out using putida and . These PHAs were produced using reagent-grade glycerol and crude glycerol as the carbon sources. The objective was to compare the production of PHAs and to functionalize these polymers with silver nanoparticles to provide antibacterial properties for potential biomedical applications. The findings from the physical and chemical analyses confirmed the successful synthesis and extraction of PHAs, achieving comparable yields using both crude glycerol and reagent-grade glycerol as carbon sources across both strains. Approximately 16% higher PHAs production was obtained using compared to , and no significant difference was observed in the production rate of PHAs between the two carbon sources used, which means that crude glycerol could be utilized even though it has more impurities. Notably, PHAs functionalized with silver nanoparticles showed improved antibacterial effectiveness, especially those derived from reagent-grade glycerol and the strain.
在这项研究中，使用 和 来进行聚羟基烷酸酯（PHA）的生物合成。这些 PHA 是使用试剂级甘油和粗甘油作为碳源生产的。目的是比较 PHA 的生产，并对这些聚合物进行功能化，以赋予其银纳米粒子的抗菌性能，从而为潜在的生物医学应用提供抗菌性能。物理化学分析的结果证实了 PHA 的成功合成和提取，使用两种碳源（粗甘油和试剂级甘油）在两种菌株中均获得了相当的产率。与 相比，使用 可获得约 16%更高的 PHA 产量，而两种所用碳源的 PHA 生产速率没有明显差异，这意味着即使粗甘油含有更多杂质，也可以加以利用。值得注意的是，用银纳米粒子功能化的 PHA 显示出增强的抗菌效果，特别是那些源自试剂级甘油和 菌株的 PHA。
