Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Molecules. 2024 Aug 20;29(16):3925. doi: 10.3390/molecules29163925.
Bone grafting is crucial for bone regeneration. Recent studies have proposed the use of calcium citrate (CC) as a potential graft material. Notably, citrate does not inhibit hydroxyapatite (HAp) formation at specific calcium-to-citrate molar ratios. Octacalcium phosphate (OCP)/gelatine (Gel) composites, which are commonly produced from porcine Gel, are valued for their biodegradability and bone replacement capability. This study introduces fish Gel as an alternative to porcine Gel because of its wide acceptance and eco-friendliness. This is the first study to examine the interaction effects between two osteogenic materials, OCP/CC, and the influence of different gelatine matrix components on HAp formation in an SBF. Samples with varying CC contents were immersed in an SBF for 7 d and analysed using various techniques, confirming that high CC doses prevent HAp formation, whereas lower doses facilitate it. Notably, small-sized OCP/CC/porcine Gel composites exhibit a high HAp generation rate. Porcine Gel composites form denser HAp clusters, whereas fish Gel composites form larger spherical HAps. This suggests that lower CC doses not only avoid inhibiting HAp formation but also enhance it with the OCP/Gel composite. Compared with porcine Gel, fish Gel composites show less nucleation but an increased crystal growth for HAp.
骨移植对于骨再生至关重要。最近的研究提出了使用柠檬酸钙 (CC) 作为潜在的移植材料。值得注意的是，在特定的钙与柠檬酸摩尔比下，柠檬酸不会抑制羟基磷灰石 (HAp) 的形成。八钙磷酸盐 (OCP)/明胶 (Gel) 复合材料通常由猪明胶制成，因其可生物降解性和骨替代能力而受到重视。由于其广泛的接受度和生态友好性，本研究将鱼明胶作为猪明胶的替代品。这是首次研究两种成骨材料 OCP/CC 之间的相互作用效应以及不同明胶基质成分对 SBF 中 HAp 形成的影响。将具有不同 CC 含量的样品浸入 SBF 中 7 天，并使用各种技术进行分析，证实高 CC 剂量会阻止 HAp 的形成，而低剂量则会促进其形成。值得注意的是，小尺寸的 OCP/CC/猪明胶复合材料具有较高的 HAp 生成速率。猪明胶复合材料形成更密集的 HAp 簇，而鱼明胶复合材料形成更大的球形 HAp。这表明，低 CC 剂量不仅避免了抑制 HAp 的形成，而且还通过 OCP/Gel 复合材料增强了其形成。与猪明胶相比，鱼明胶复合材料的 HAp 成核较少，但晶体生长增加。
