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γ射线辐照对愈伤组织化学成分变化及抗氧化活性的影响

Effects of Gamma Irradiation on Changes in Chemical Composition and Antioxidant Activity of Callus.

作者信息

Jeong Gyeong Han, Kaur Shubhpreet, Yoo Youngchul, Ryu Young Bae, Lee Seo Jun, Jung Kwang-Woo, Chung Moon-Soo, Bai Hyoung-Woo, Kim Jin-Hong, Lee Sungbeom, Kim Tae Hoon, Chung Byung Yeoup, Lee Seung Sik

机构信息

Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, Republic of Korea.

Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Republic of Korea.

出版信息

Plants (Basel). 2024 Aug 19;13(16):2306. doi: 10.3390/plants13162306.

DOI:10.3390/plants13162306
PMID:39204742
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11360754/
Abstract

In this study, we investigated the effects of gamma irradiation on the antioxidant activity and metabolite profiles of calli (PC3012). Gamma irradiation at various doses (0, 0.05, 0.5, and 10 kGy) significantly enhanced the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities of the callus extracts of PC3012 in a dose-dependent manner. High-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) analyses revealed that irradiation increased the lysophospholipid content, although no new antioxidant compounds were formed. Furthermore, a PLS-DA analysis revealed evident metabolic differences between non-irradiated and irradiated samples, which were further verified by statistical validation. These findings suggest that gamma irradiation induces specific biochemical modifications that enhance the bioactive properties of PC3012 calli. This technology exhibits potential for utilization in the natural product and food sectors, particularly in the development of functional foods and nutraceuticals with improved health benefits.

摘要

在本研究中，我们调查了γ射线辐照对愈伤组织（PC3012）抗氧化活性和代谢物谱的影响。不同剂量（0、0.05、0.5和10千戈瑞）的γ射线辐照以剂量依赖的方式显著增强了PC3012愈伤组织提取物的1,1-二苯基-2-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性。高效液相色谱（HPLC）和超高效液相色谱-四极杆飞行时间/质谱（UPLC-Q-TOF/MS）分析表明，辐照增加了溶血磷脂含量，尽管没有形成新的抗氧化化合物。此外，偏最小二乘判别分析（PLS-DA）显示未辐照和辐照样品之间存在明显的代谢差异，这通过统计验证得到了进一步证实。这些发现表明，γ射线辐照诱导了特定的生化修饰，增强了PC3012愈伤组织的生物活性特性。该技术在天然产物和食品领域具有应用潜力，特别是在开发具有更好健康益处的功能性食品和营养保健品方面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/9fe00d861549/plants-13-02306-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/f613a535c00c/plants-13-02306-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/d88469ffea7f/plants-13-02306-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/410644c5ebbf/plants-13-02306-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/f3de20e8fa72/plants-13-02306-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/cd696b81cf51/plants-13-02306-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/9fe00d861549/plants-13-02306-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/f613a535c00c/plants-13-02306-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/d88469ffea7f/plants-13-02306-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/410644c5ebbf/plants-13-02306-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/f3de20e8fa72/plants-13-02306-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/cd696b81cf51/plants-13-02306-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f7/11360754/9fe00d861549/plants-13-02306-g006.jpg

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