Wood C R, Boss M A, Kenten J H, Calvert J E, Roberts N A, Emtage J S
Nature. 1985;314(6010):446-9. doi: 10.1038/314446a0.
The yeast Saccharomyces cerevisiae can synthesize, process and secrete higher eukaryotic proteins. We have investigated the expression of immunoglobulin chains in yeast and demonstrate here the synthesis, processing and secretion of light and heavy chains, the glycosylation of heavy chain, the intracellular localization of these foreign proteins by immunofluorescence, and the detection of functional antibodies in cells co-expressing both chains. This may provide the basis of a microbial fermentation process for the production of monoclonal antibodies. The co-expression of light and heavy chains in Escherichia coli has been reported but functional antibodies were not assembled in vivo. Furthermore, only low-level assembly of these chains was found in vitro.
酿酒酵母能够合成、加工并分泌高等真核生物的蛋白质。我们研究了酵母中免疫球蛋白链的表达情况，在此展示了轻链和重链的合成、加工及分泌过程，重链的糖基化，通过免疫荧光法对这些外源蛋白进行细胞内定位，以及在共表达两条链的细胞中检测功能性抗体。这可能为生产单克隆抗体的微生物发酵工艺提供基础。已有报道称在大肠杆菌中共表达轻链和重链，但功能性抗体在体内并未组装成功。此外，在体外仅发现这些链的低水平组装。
