小鼠重链可变区：种系VH基因片段的核苷酸序列
Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
作者信息
Ollo R, Auffray C, Sikorav J L, Rougeon F
出版信息
Nucleic Acids Res. 1981 Aug 25;9(16):4099-109. doi: 10.1093/nar/9.16.4099.
Abstract
We have constructed a library of Balb/c mouse embryo DNA in the vector Charon 4A. The library was searched for sequences homologous to the VH region of a cloned cDNA of the UPC10 heavy chain mRNA. In this paper, we describe the structure and the partial nucleotide sequence of one of such clones (VH441). The nucleotide sequence of this germ-line gene indicates that it encodes amino-acids 1-98 of the X44 and J601 galactan-binding VH regions, but that it differs from the UPC10 VH segment by four single base changes. The VH gene appears to contain a 101 bases long intervening sequence within a precursor sequence identical to the precursor sequence of UPC10. The 3' non coding sequence of the V gene contains the two conserved sequences found in embryonic V DNA segments, CACAGTG and ACATGAACC, separated by 23 nucleotides and a sequence CACTGTG separated by 33 nucleotides from the first heptamer.
摘要
我们构建了一个以Charon 4A为载体的Balb/c小鼠胚胎DNA文库。在该文库中搜索与UPC10重链mRNA克隆cDNA的VH区域同源的序列。在本文中，我们描述了其中一个这样的克隆（VH441）的结构和部分核苷酸序列。这个种系基因的核苷酸序列表明，它编码X44和J601半乳聚糖结合VH区域的第1至98个氨基酸，但与UPC10 VH片段有四个单碱基变化。该VH基因似乎在前体序列中包含一个101个碱基长的间隔序列，该前体序列与UPC10的前体序列相同。V基因的3'非编码序列包含在胚胎V DNA片段中发现的两个保守序列，即CACAGTG和ACATGAACC，它们相隔23个核苷酸，还有一个CACTGTG序列，与第一个七聚体相隔33个核苷酸。
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