Chen Yanhong, Zhang Jian
School of Life Sciences, Nantong University, Nantong, China; Key Laboratory of Landscape Plant Genetics and Breeding, Nantong, China.
School of Life Sciences, Nantong University, Nantong, China; Key Laboratory of Landscape Plant Genetics and Breeding, Nantong, China.
Gene. 2024 Dec 30;931:148899. doi: 10.1016/j.gene.2024.148899. Epub 2024 Aug 28.
Arabidopsis thaliana WRKY33 is currently one of the most studied members of the Group I WRKY transcription factor family. Research has confirmed that WRKY33 is involved in the regulation of various biological and abiotic stresses and occupies a central position in the regulatory network. The functional studies of orthologous genes of WRKY33 from other species are also receiving increasing attention. In this article, we summarized thirty-eight orthologous genes of AtWKRY33 from twenty-five different species. Their phylogenetic relationship and conserved WRKY domain were analyzed and compared. Similar to AtWKRY33, the well-studied orthologous gene members from rice and tomato also have multiple functions. In addition to playing important regulatory roles in responding to their specific pathogens, they are also involved in regulating various abiotic stresses and development. AtWKRY33 exerts its multiple functions through a complex regulatory network. Upstream transcription factors or other regulatory factors activate or inhibit the expression of AtWKRY33 at the chromatin and transcriptional levels. Interacting proteins affect the transcriptional activity of AtWKRY33 through phosphorylation, ubiquitination, SUMOylation, competition, or cooperation. The downstream genes are diverse and include three major categories: transcription factors, synthesis, metabolism, and signal transduction of various hormones, and disease resistance genes. In the regulatory network of AtWRKY33 orthologs, many conserved regulatory characteristics have been discovered, such as self-activation and phosphorylation by MAP kinases. This can provide a comparative reference for further studying the functions of other orthologous genes of AtWKRY33.
拟南芥WRKY33是目前I类WRKY转录因子家族中研究最多的成员之一。研究证实，WRKY33参与多种生物和非生物胁迫的调控，在调控网络中占据核心地位。来自其他物种的WRKY33直系同源基因的功能研究也越来越受到关注。在本文中，我们总结了来自25个不同物种的38个AtWKRY33直系同源基因。分析并比较了它们的系统发育关系和保守的WRKY结构域。与AtWKRY33类似，水稻和番茄中研究充分的直系同源基因成员也具有多种功能。它们除了在应对特定病原体方面发挥重要调控作用外，还参与调控各种非生物胁迫和发育过程。AtWKRY33通过复杂的调控网络发挥其多种功能。上游转录因子或其他调控因子在染色质和转录水平激活或抑制AtWKRY33的表达。相互作用蛋白通过磷酸化、泛素化、SUMO化、竞争或合作影响AtWKRY33的转录活性。下游基因多种多样，包括三大类：转录因子、各种激素的合成、代谢和信号转导，以及抗病基因。在AtWRKY33直系同源基因的调控网络中，发现了许多保守的调控特征，如自激活和被MAP激酶磷酸化。这可以为进一步研究AtWKRY33其他直系同源基因的功能提供比较参考。
