Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.
Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Methods Mol Biol. 2024;2851:213-226. doi: 10.1007/978-1-0716-4096-8_19.
Microorganisms with the ability to modulate the immune system (immunobiotics) have shown to interact with different pattern recognition receptors (PRRs) expressed in nonimmune and immune cells and exert beneficial effects on host's health maintenance and promotion. Suitable assay systems are necessary for an efficient and rapid screening of potential immunobiotic strains. More than a decade of research has allowed us to develop efficient in vitro models based on porcine receptors and cells (porcine immunoassay systems) to study the immunomodulatory effects of lactic acid bacteria (LAB). In addition, detailed studies of model immunobiotic LAB strains with proved abilities to improve immune health in humans (Lactobacillus rhamnosus CRL1505) or pigs (Lactobacillus jensenii TL2937) allowed us to select the most suitable biomarkers that have to be evaluated in those porcine immunoassay systems. Our in vitro models, utilizing transfectant cells expressing PRRs along with an established porcine intestinal epitheliocyte (PIE) cell line, have proven to be valuable tools for immunobiotic selection and for gaining insights into the molecular mechanisms responsible for their beneficial effects.
具有调节免疫系统能力的微生物（免疫生物）已被证明可以与非免疫和免疫细胞中表达的不同模式识别受体（PRRs）相互作用，并对宿主的健康维护和促进产生有益影响。合适的测定系统对于有效和快速筛选潜在的免疫生物菌株是必要的。十多年的研究使我们能够开发基于猪受体和细胞（猪免疫测定系统）的高效体外模型，以研究乳酸菌（LAB）的免疫调节作用。此外，对具有证明能够改善人类（鼠李糖乳杆菌 CRL1505）或猪（詹氏乳杆菌 TL2937）免疫健康能力的模型免疫生物 LAB 菌株的详细研究使我们能够选择最适合的生物标志物，这些标志物必须在那些猪免疫测定系统中进行评估。我们的体外模型利用表达 PRRs 的转染细胞以及已建立的猪肠上皮细胞（PIE）系，已被证明是免疫生物选择的有价值工具，并深入了解其有益作用的分子机制。
