Morgan A F, Dean H F
J Gen Microbiol. 1985 Apr;131(4):885-96. doi: 10.1099/00221287-131-4-885.
The generalized transducing phage Pf16h2 has been used to confirm linkage relationships of chromosomal markers of Pseudomonas putida previously determined from their time-of-entry in Hfr crosses, and to map new auxotrophic mutations. By means of spot matings using Hfr donors of known origin of transfer, catabolic markers forming part of a closely linked group of operons referred to as a superoperonic cluster have been shown to be chromosomally located and their map positions determined. R-prime-mediated interspecific complementation has been used to equate functionally 21 auxotrophic loci in P. putida and P. aeruginosa, and the distribution of these loci on the two genetic maps has been compared. While both maps reveal that auxotrophic markers are largely restricted to about 40% of the chromosome and that auxotrophic markers of similar phenotype are not clustered, there is evidence of at least seven chromosomal rearrangements since divergence from a presumed common ancestor.
广义转导噬菌体Pf16h2已被用于确认恶臭假单胞菌染色体标记的连锁关系，这些关系先前已通过它们在高频重组（Hfr）杂交中的进入时间确定，并且用于定位新的营养缺陷型突变。通过使用已知转移起点的Hfr供体进行斑点杂交，已证明构成紧密连锁的操纵子组（称为超级操纵子簇）一部分的分解代谢标记位于染色体上，并确定了它们的图谱位置。R-prime介导的种间互补已用于在功能上对等恶臭假单胞菌和铜绿假单胞菌中的21个营养缺陷型位点，并比较了这些位点在两个遗传图谱上的分布。虽然两个图谱都显示营养缺陷型标记主要局限于约40%的染色体，并且相似表型的营养缺陷型标记没有聚集，但有证据表明自假定的共同祖先分化以来至少发生了七次染色体重排。
