Watson J M, Holloway B W
J Bacteriol. 1978 Nov;136(2):507-21. doi: 10.1128/jb.136.2.507-521.1978.
The locations of new markers relative to markers previously mapped on the chromosome of Pseudomonas aeruginosa strain PAT were defined by generalized transduction with phage F116L and F1083. Although the marker orders of the various marker groups were deduced mainly from the results of two-factor crosses, the locations of a number of markers were confirmed by three-factor crosses. A linkage map of the chromosome of P. aeruginosa PAT was constructed which shows the relative locations of 50 genes. From the available data, the linkage maps of P. aeruginosa strains PAO and PAT appear to be similar.
通过用噬菌体F116L和F1083进行广义转导,确定了新标记相对于先前定位在铜绿假单胞菌菌株PAT染色体上的标记的位置。虽然各个标记组的标记顺序主要是从双因子杂交结果推导出来的,但一些标记的位置通过三因子杂交得到了证实。构建了铜绿假单胞菌PAT染色体的连锁图谱,该图谱显示了50个基因的相对位置。根据现有数据,铜绿假单胞菌菌株PAO和PAT的连锁图谱似乎相似。