Debruyne D, Moulin M A, Bricard H, Bigot M C
J Pharm Sci. 1985 Feb;74(2):224-6. doi: 10.1002/jps.2600740227.
A high-performance liquid chromatographic method is described which determines noxytiolin and 1-methyl-2-thiourea concentrations in serum. Valid determination requires immediate ultracentrifugation of blood samples, rapid serum freezing, and injection into the chromatograph within 6 h. A number of pharmacokinetic parameters were calculated from serum concentration data in rabbits and humans. An unknown metabolite was detected in both species but its structure was not identified.
本文描述了一种高效液相色谱法，用于测定血清中的诺西替林和1-甲基-2-硫脲浓度。有效的测定需要对血样立即进行超速离心、快速冷冻血清，并在6小时内注入色谱仪。根据兔和人的血清浓度数据计算了一些药代动力学参数。在两个物种中均检测到一种未知代谢物，但其结构未确定。
