Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian 271018, China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian 271018, China.
Poult Sci. 2024 Nov;103(11):104227. doi: 10.1016/j.psj.2024.104227. Epub 2024 Aug 20.
Salmonella is a globally prevalent foodborne pathogen, and adverse events caused by S. Enteritidis and S. Typhimurium are extremely common. With the emergence of drug resistance, there is an urgent need for efficient and specific lytic bacteriophages as alternative to antibiotics in clinical practice. In this study, phage P6 was isolated and screened from effluent and fecal samples from duck farm environments to specifically lyse the duck sources S. Typhimurium and S. Enteritidis. Phage P6 belongs to the genus Lederbergvirus, unclassified Lederbergvirus species. The phage P6 genome did not contained non-coding RNA, virulence genes and drug resistance genes, indicating that phage P6 was biologically safe for clinical applications. Phage P6 lysed 77.78% (28/36) of multidrug-resistant Salmonella and reduced biofilms formed by S. Enteritidis CVCC 3377, 4, and 24, and S. Typhimurium 44 by 44% to 75% within 3 h, and decreased Salmonella in duckling feces by up to 1.64 orders of magnitude. Prokaryotic expression of endolysin LysP6 lysed the chloroform-treated bacterial outer membrane from different serotypes of duck-derived Salmonella and E. coli standard strain ATCC 25922. The host range was expanded compared to phage P6, and the growth of Salmonella was effectively inhibited by LysP6 in conjunction with the membrane permeabilizer EDTA within 24 h. Therefore, phage P6 and phage-derived endolysins LysP6 are suitable for application as potent biocontrol agents to improve poultry health and food safety.
肠炎沙门氏菌是一种全球性流行的食源性病原体，由肠炎沙门氏菌和鼠伤寒沙门氏菌引起的不良事件极其常见。随着耐药性的出现，迫切需要有效的、特异性的裂解噬菌体作为抗生素在临床实践中的替代品。在本研究中，从鸭场环境的污水和粪便样本中分离和筛选出噬菌体 P6，以特异性裂解鸭源鼠伤寒沙门氏菌和肠炎沙门氏菌。噬菌体 P6 属于 Lederbergvirus 属，未分类的 Lederbergvirus 种。噬菌体 P6 基因组不包含非编码 RNA、毒力基因和耐药基因，表明噬菌体 P6 对临床应用具有生物安全性。噬菌体 P6 可裂解 77.78%（28/36）的多药耐药性沙门氏菌，并在 3 小时内将肠炎沙门氏菌 CVCC 3377、4 和 24 以及鼠伤寒沙门氏菌 44 的生物膜减少 44%至 75%，并使雏鸭粪便中的沙门氏菌减少多达 1.64 个数量级。内溶素 LysP6 的原核表达可裂解来自不同鸭源沙门氏菌血清型和大肠杆菌标准株 ATCC 25922 的氯仿处理细菌外膜。与噬菌体 P6 相比，宿主范围扩大了，并且 LysP6 与膜通透剂 EDTA 联合使用可在 24 小时内有效抑制沙门氏菌的生长。因此，噬菌体 P6 和噬菌体衍生的内溶素 LysP6 适合作为有效的生物防治剂应用，以改善家禽健康和食品安全。
