Li Zhangjie, Yu Dingyuan, Zhou Chenyang, Wang Feifan, Lu Kangyi, Liu Yijun, Xu Jiaqi, Xuan Lian, Wang Xiaolin
Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Institute of Medical Robotics, Shanghai Jiao Tong University, Shanghai, China.
Biomater Transl. 2024 Mar 28;5(1):21-32. doi: 10.12336/biomatertransl.2024.01.003. eCollection 2024.
In recent years, advances in microfabrication technology and tissue engineering have propelled the development of a novel drug screening and disease modelling platform known as organoid-on-a-chip. This platform integrates organoids and organ-on-a-chip technologies, emerging as a promising approach for in vitro modelling of human organ physiology. Organoid-on-a-chip devices leverage microfluidic systems to simulate the physiological microenvironment of specific organs, offering a more dynamic and flexible setting that can mimic a more comprehensive human biological context. However, the lack of functional vasculature has remained a significant challenge in this technology. Vascularisation is crucial for the long-term culture and in vitro modelling of organoids, holding important implications for drug development and personalised medical approaches. This review provides an overview of research progress in developing vascularised organoid-on-a-chip models, addressing methods for in vitro vascularisation and advancements in vascularised organoids. The aim is to serve as a reference for future endeavors in constructing fully functional vascularised organoid-on-a-chip platforms.
近年来，微制造技术和组织工程学的进展推动了一种名为“芯片上类器官”的新型药物筛选和疾病建模平台的发展。该平台整合了类器官和芯片上器官技术，成为体外模拟人体器官生理学的一种有前景的方法。芯片上类器官装置利用微流体系统来模拟特定器官的生理微环境，提供了一个更具动态性和灵活性的环境，能够模拟更全面的人类生物学背景。然而，缺乏功能性血管仍然是该技术面临的重大挑战。血管化对于类器官的长期培养和体外建模至关重要，对药物开发和个性化医疗方法具有重要意义。本综述概述了开发血管化芯片上类器官模型的研究进展，阐述了体外血管化方法和血管化类器官的进展。目的是为未来构建功能齐全的血管化芯片上类器官平台的努力提供参考。
